食品科学 ›› 2009, Vol. 30 ›› Issue (22 ): 37-39.doi: 10.7506/spkx1002-6300-200922004

• 工艺技术 • 上一篇    下一篇

利用悬浮细胞培养方法提高荞麦中总黄酮含量的研究

于寒松,于亚桐,贾 帅,朴春红,胡耀辉*   

  1. 吉林农业大学食品科学与工程学院
  • 收稿日期:2009-06-02 出版日期:2009-11-15 发布日期:2010-12-29
  • 通讯作者: 胡耀辉* E-mail:huyaohui@vip.163.com
  • 基金资助:

    农业部“948”项目(2008-Z27)

Enhanced Accumulation of Total Flavonoids in Buckwheat Subjected to Cell Suspension Culture

YU Han-song,YU Ya-tong,JIA Shuai,PIAO Chun-hong,HU Yao-hui*   

  1. (College of Food Science and Engineering, Jilin Agricultural University, Jilin 130118, China)
  • Received:2009-06-02 Online:2009-11-15 Published:2010-12-29
  • Contact: HU Yao-hui* E-mail:huyaohui@vip.163.com

摘要:

利用植物组织培养方法对荞麦不同组织进行愈伤诱导实验,进一步建立荞麦悬浮培养体系,并对其黄酮含量进行比较分析。结果表明,利用荞麦叶柄部分获得的荞麦愈伤组织总黄酮含量较高,达到58.80mg/g,分别是荞麦叶柄总黄酮含量和荞麦茎段总黄酮含量的3.1 倍和3.7 倍,而荞麦细胞悬浮培养物中总黄酮含量可达90.6mg/g,是荞麦叶柄愈伤组织含量的1.54 倍。

关键词: 组织培养, 微波, 超声波

Abstract:

Buckwheat stem and leafstalk explants were subjected to callus induction and cell suspension culture. The accumulation of total flavonoids in various explants during the cell suspension culture was compared. Results showed that buckwheat leafstalk callus revealed a total flavonoids content of 58.80 mg/g, which was 3.1 and 3.7 times higher than that in buckwheat leafstalk and stem, respectively, and the total flavonoids content in cell suspension cultures was as high as 90.6 mg/g, which was 1.54 times higher than that in buckwheat leafstalk callus.

Key words: tissue culture, microwave, ultrasonic

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