关键词: 乳酸菌, cDNA 微阵列, 应激代谢, 转录组特征

Abstract:

A local MySQL database of lactic acid bacteria (LAB) was built through integrating genome data and chip data from gene expression profile used perl program written by our laboratory. The local database was used to investigate transcriptome properties of LAB in different growth phases or stress environments such as the presence of bile salts, hydrogen peroxide and starvation. Meanwhile, MEME software was also used for searching the common motif located in upstream of transcription starting point in co-expression genes under certain stress environments. Results indicated that no obvious difference was observed in transcriptome properties of LAB under the stress from bile and ammonium oxalate. However, a significant difference was observed in transcriptome properties of LAB under the stress from other factors. These responses to stress exhibited specific induced or inhibition gene expression. In addition, gene expression associated with translation (J), lipid transport and metabolism (I), intracellular transport and secretion (U) revealed a high level, especially for translation-related genes, which indicating that modulation of cell metabolism was mainly achieved through regulating the expression of translation-related genes.

Key words: lactic acid bacteria, cDNA microarray, stress response, transcriptome property