食品科学 ›› 2010, Vol. 31 ›› Issue (5): 104-107.doi: 10.7506/spkx1002-6300-201005024

• 基础研究 • 上一篇    下一篇

荔枝皮色素体外清除亚硝酸盐作用研究

宋茹1,韦荣编2,胡金申1,周桂晓1   

  1. 1. 浙江海洋学院食品与药学学院 2. 浙江海洋学院海洋科学学院
  • 收稿日期:2009-07-13 出版日期:2010-03-01 发布日期:2010-12-29
  • 通讯作者: 宋茹 E-mail:happysong545@yahoo.com.cn

in vitro Nitrite Scavenging Capacity of Litchi Pericarp Pigment

SONG Ru1,WEI Rong-bian2,HU Jin-shen1,ZHOU Gui-xiao1   

  1. 1. College of Food Science and Pharmacy, Zhejiang Ocean University, Zhoushan 316004, China;
    2. School of Marine Science, Zhejiang Ocean University, Zhoushan 316004, China
  • Received:2009-07-13 Online:2010-03-01 Published:2010-12-29
  • Contact: SONG Ru E-mail:happysong545@yahoo.com.cn

摘要:

采用分光光度法,测定荔枝皮色素体外清除亚硝酸盐能力,并与茶叶提取液和VC 对亚硝酸盐的体外清除作用进行比较。结果表明,荔枝皮色素同亚硝酸盐反应10min,对亚硝酸盐的清除基本完全,亚硝酸盐的清除率随荔枝皮色素添加量的增加先增大而后趋于稳定。加热处理、反应体系酸性pH 值能有效增强荔枝皮色素对亚硝酸盐的清除效果。100℃加热10min 条件下,1mg/mL 和10mg/mL 的VC 对亚硝酸盐清除率均最高,荔枝皮色素在低质量浓度(1mg/mL)时对亚硝酸盐清除率为(42.76±0.98)%,高于同质量浓度的茶叶提取液对亚硝酸盐的清除率((37.92±0.79)%)(P < 0.05),但在高质量浓度(10mg/mL)下对亚硝酸盐的清除率则低同质量浓度的茶叶提取液(P < 0.05)。体外模拟胃酸和体温条件下,荔枝皮色素保持较高的亚硝酸盐清除效果,1mg/mL 和10mg/mL 时的清除率分别为(23.55 ± 0.45)% 和(51.62 ± 0.91)%。

关键词: 荔枝皮, 色素, 亚硝酸盐, 清除作用

Abstract:

The nitrite scavenging capacity of litchi pericarp pigment was evaluated in vitro by spectrophotometric method and compared with those of tea extract and vitamin C. Results indicated that the reaction between litchi pericarp pigment and nitrite for 10 min resulted in perfect scavenging of nitrite. The scavenging rate against nitrite first increased and then tended to keep stable with increasing dose of litchi pericarp pigment. Heating treatment and low pH of reaction system could improve the scavenging effect against nitrite. As a result of heating treatment at 100 ℃ for 10 min, the nitrite scavenging rates of vitamin C at the concentration levels of 1 and 10 mg/mL were both the highest. Moreover, the nitrite scavenging rate of litchi pericarp pigment at the low concentration level of 1 mg/mL was (42.76 ± 0.98)%, which was higher than that of tea extract ((37.92 ± 0.79)%) (P<0.05) at the identical concentration level; while the nitrite scavenging rate of tea extract was higher than that of litchi pericarp pigment at the high concentration level of 10 mg/mL (P<0.05). Furthermore, high nitrite scavenging rate of litchi pericarp pigment was also observed under simulated gastric and body temperature conditions in vitro, with the values of (23.55± 0.45)% and (51.62± 0.91)% at the concentration levels of 1 and 10 mg/mL, respectively.

Key words: litchi pericarp, pigment, nitrite, scavenging rate

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