食品科学 ›› 2010, Vol. 31 ›› Issue (5): 194-196.doi: 10.7506/spkx1002-6300-201005043

• 生物工程 • 上一篇    下一篇

发酵乳杆菌增殖培养基营养因子优化研究

叶雪飞,阮 辉,冯石开,朱承亮,何国庆*   

  1. 浙江大学生物系统工程与食品科学学院
  • 收稿日期:2008-05-15 修回日期:2009-11-27 出版日期:2010-03-01 发布日期:2010-12-29
  • 通讯作者: 何国庆 E-mail:gqhe@zju.edu.cn
  • 基金资助:

    浙江省科技计划项目(2006C12095)

Optimization of Nutrient Factors in Medium for Lactobacillus fermentum Growth

YE Xue-fei,RUAN Hui,FENG Shi-kai,ZHU Cheng-liang,HE Guo-qing*   

  1. School of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou 310029, China
  • Received:2008-05-15 Revised:2009-11-27 Online:2010-03-01 Published:2010-12-29
  • Contact: HE Guo-qing E-mail:gqhe@zju.edu.cn

摘要:

以MRS 培养基为基础,选择碳源、氮源、生长因子这3 个主要的营养成分进行单因素试验,然后利用L9(34)正交试验优化出发酵乳杆菌增殖培养基营养因子最佳组成为:葡萄糖2.0%,牛肉膏0.5%,胰酶解酪朊0.5%,玉米浆2%,其他MRS 基本成分保持不变。研究结果表明,发酵乳杆菌La-Y1 在优化后的MRS 培养基中,37℃培养16~18h,菌落数高于用原始MRS 培养的发酵液,达到了1010CFU/mL 以上。

关键词: 发酵乳杆菌La-Y1, 营养因子, 增殖培养基

Abstract:

In this study, the optimal basal medium and the optimal carbon source, nitrogen source and growth factor in it were chosen for Lactobacilus fermentum growth, and the optimal addition amounts of the three nutrient factors were investigated using orthogonal array design. Results indicated that MRS medium was the most suitable for Lactobacilus fermentum growth, and its optimal formula consisted of 2.0% glucose, 0.5% beef extract, 0.5% casein peptone, 2% corn syrup, and other unchanged basic ingredients. The number of living Lactobacilus fermentum cells was over 1010 CFU/mL after 16 h to 18 h of cultivation in optimized MRS medium at 37 ℃, which exhibited a 10-fold increase compared with that in original MRS medium.

Key words: Lactobacilus fermentum La-Y1, nutrient factor, growth culture

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