关键词: 阿魏菇, 阿魏萃取物, 漆酶, PPO, 蛋白酶

Abstract:

In this study, Pleurotus ferulae NB-1 was used for liquid fermentation. The chloroform soluble fraction of 95% ethanol extract from Ferula ferulaeoides root was added to fermentation culture medium of the strain. After 7 days of fermentation at 27.5 ℃, the resulting fermentation broth was harvested and separated into supernatant and mycelia by centrifugation, and the activities of laccase, polyphenol oxidase (PPO) and protease in the supernatant and mycelial PPO were determined. Results indicated that the addition of the chloroform soluble fraction of 95% ethanol extract from Ferula ferulaeoides root at the levels of 10, 50 and 100 mg to 100 mL of fermentation culture medium resulted in 40-, 50- and 50-fold increases of laccase activity, 4.43-, 30.14- and 43.14-fold increases of PPO activity and 0.99-, 0.85- and 0.67-fold increase of protease in the supernatant, and 1.6-, 0.93- and 1.06-fold increases of mycelial PPO activity, respectively when compared with no addition. These results demonstrate that the chloroform soluble fraction of 95% ethanol extract from Ferula ferulaeoides root exhibits a promotion effect at low concentration and an inhibition effect on PPO activity at high concentration. Moreover, protease activity exhibits a decreasing trend in a dosedependent manner.

Key words: Pleurotus ferulae , chloroform soluble fraction of 95% ethanol extract from Ferula ferulaeoides root , laccase , polyphenol oxidase , protease