关键词: 肥肝, 脂肪酸, 毛细管气相色谱法, VE, 饲粮油脂

Abstract:

Fatty acids in fatty liver of cherry valley duck were extracted from tissue using reflux with “Folch” solvent, and esterified with KOH-BF3 method followed by gas chromatography analysis. A fused-silica BPX70 capillary column and FID detector were employed in gas chromatography. The inlet mode was split with split ratio of 100:1. Temperature program settings were as following：initial temperature 220 ℃，increase at 1 ℃/min to final temperature 240 ℃. 11 kinds of fatty acids in fatty liver were identified by applying linear programmed temperature equivalent chain length (ECLT) values based on even-chain saturated fatty acid methyl ester standards. Area normalization method could be used to quantify the content of fatty acids and investigate the effects of camellia seed oil, sesame oil, soybean oil and vitamin E supplement on fatty acids in fatty liver production. The results indicated that: 2% of 3 dietary oil supplements significantly decrease the contents of saturated fatty acid(p＜0.01) and significantly increase the contents of unsaturated fatty acid (p ＜ 0.01); 90 IU/kg of vitamin E supplement appeares to significantly decrease the contents of saturated fatty acid (p＜0.05) and increases the contents of unsaturated fatty acid (p＜ 0.05).

Key words: fatty liver, fatty acid, capillary gas chromatography, vitamin E, dietary oil