食品科学 ›› 2009, Vol. 30 ›› Issue (10): 131-134.doi: 10.7506/spkx1002-6630-200910025

• 工艺技术 • 上一篇    下一篇

石榴叶酚酸测定及提取工艺研究

邱涛涛1,王华2,毛世红1   

  1. 1.西南大学食品科学学院 2.中国农业科学院柑桔研究所
  • 收稿日期:2008-11-07 修回日期:2009-01-18 出版日期:2009-05-15 发布日期:2010-12-29
  • 通讯作者: 王华 E-mail:wanghua40·126.com

Study on Determination and Extraction of Total Polyphenols from Leaves of Punica granatum L.

QIU Tao-tao1,WANG Hua2,*,MAO shi-hong1   

  1. (1. College of Food Science, Southwest University, Chongqing 400716, China;
    2. Citrus Research Institute, Chinese Academy of Agricultural Sciences, Chongqing 400712, China)
  • Received:2008-11-07 Revised:2009-01-18 Online:2009-05-15 Published:2010-12-29
  • Contact: WANG Hua2,*, E-mail:wanghua40·126.com

摘要:

以石榴叶为原料,采用Folin-Ciocalteu 法定量测定石榴叶提取液中总酚含量,通过单因素试验和正交试验对石榴叶总酚的提取工艺进行探讨。确定其最佳提取条件为:料液比1:25(g/ml),温度70℃,提取溶剂为75% 乙醇,提取时间1.5h。综合考虑成本因素,原料过1mm 筛较为适宜,按照该工艺提取石榴叶总酚的得率为16.63%。

关键词: 石榴叶, 总酚, Folin-Ciocalteu法, 提取

Abstract:

Folin-Ciocalteu method was used to determine the content of total polyphenols from leaves of Punica granatum L.. The effect of extraction conditions on the extraction rate of total polyphenols from extracts was evaluated by single factor and orthogonal tests. The optimal extraction conditions of total polyphenols from leaves of Punica granatum L. are as follows: 75% alcohol as extraction solvent, temperature 70 ℃, extraction time1.5 h, material to liquid ratio 1:20 and 1 mm of particle size. Under these conditions, the maximum extraction rate of total polyphenols from leaves of Punica granatum L. was 16.63%.

Key words: leaves of Punica granatum L., total polyphenols, Folin-Ciocalteu, extraction

中图分类号: