关键词: 液相色谱, 食品, 高效氯氟氰菊酯, 快速检测

Abstract:

A HPLC method was developed for the analysis of lambda-cyhalothrin residue in drinking water (from polluted rivers) and Brassica chinensis leaves. The analysis was performed on a SB-C18 column using acetonitrile-water (80:20, V/V) as a mobile phase at the flow rate of 1.25 ml/min and detection wavelength was set as 230 nm. Compared to other solvents, acetone-n-hexane (1:1, V/V) mixture exhibited the highest extraction efficiency to lambda-cyhalothrin (the highest recovery) from drinking water. Acetone-petroleum ether (1:1, V/V) mixture led to the highest recovery for lambda-cyhalothrin from ground Brassica chinensis leaves, and vortex-mixing extraction was adopted due to the highest the highest recovery for lambda-cyhalothrin as well as its time-saving contribution. The recoveries for lambda-cyhalothrin in drinking water and ground Brassica chinensis leaves spiked at three levels were in the rages of 97.93%－103.02% and 91.4%－113.9%, respectively, and the RSDs were less than 4%. This method is proved to be easily performed and time saving (within 30 min) and can be applied in the food safety detection.

Key words: HPLC, food, lambda-cyhalothrin, rapid determination