食品科学 ›› 2009, Vol. 30 ›› Issue (15 ): 109-113.doi: 10.7506/spkx1002-6630-200915024

• 基础研究 • 上一篇    下一篇

巴戟天多糖在离子交换填料上吸附性能的研究

何传波1,李 琳2,汤凤霞1,熊何健1   

  1. 1. 集美大学生物工程学院 2. 华南理工大学轻工与食品学院
  • 收稿日期:2009-05-06 出版日期:2009-08-01 发布日期:2010-12-29
  • 通讯作者: 何传波 E-mail:hcbcc@sina.com
  • 基金资助:

    广东省科技攻关项目(2003C104025)

Adsorption Properties of Polysaccharides from Morinda officinalis onto Ion Exchange Adsorbent

HE Chuan-bo1,LI Lin2,TANG Feng-xia1,XIONG He-jian1   

  1. 1. School of Bioengineering, Jimei University, Xiamen 361021, China;
    2. School of Light Chemistry and Food Science, South China University of Technology, Guangzhou 510640, China
  • Received:2009-05-06 Online:2009-08-01 Published:2010-12-29
  • Contact: HE Chuan-bo E-mail:hcbcc@sina.com

摘要:

研究了巴戟天多糖(polysaccharide from Morinda officinalis How,MOP)在离子交换填料DEAE Sepharose CL-6B 上的吸附行为,通过静态吸附实验考察了缓冲液的pH 值和离子强度对平衡吸附量、吸附等温线和吸附动力学的影响。结果表明,在实验浓度范围内,吸附平衡数据符合单分子层吸附的Langmuir 方程,通过拟合数据可直观地反映pH 值和离子强度对MOP 吸附的影响;MOP 在DEAE-Sepharose CL-6B 上的吸附随着缓冲液pH 值的上升而增加,低离子强度的缓冲液有利于多糖吸附。

关键词: 巴戟天, 多糖, 离子交换填料, 吸附

Abstract:

The adsorption properties of polysaccharides from Morinda officinalis (MOP) onto an ion exchange adsorbent, DEAE Sepharose CL-6B, were studied. The effects of pH and ion strength on equilibrium adsorption capacity, isotherm and dynamic curve were discussed by static adsorption experiments. The equilibrium adsorption data obtained could be described well by the Langmuir isotherm equation with high correlation coefficients, suggesting the presence of monomolecular adsorption. The fitted curves could visually demonstrate the influences of pH and ion strength on MOP adsorption onto DEAE Sepharose CL-6B. This adsorbent exhibited better adsorption performance to MOP under higher pH and lower ion strength of buffer solution.

Key words: Morinda officinalis, polysaccharides, ion exchange, adsorption

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