食品科学 ›› 2009, Vol. 30 ›› Issue (15): 169-171.doi: 10.7506/spkx1002-6630-200915038

• 生物工程 • 上一篇    下一篇

混料实验设计在纤维素酶复配中的应用研究

林增祥1,张红漫2,严立石1,陈敬文1,胡龄1,于文涛1,黄和1   

  1. 1. 南京工业大学制药与生命科学学院,材料化学工程国家重点实验室 2. 南京工业大学应用化学系
  • 收稿日期:2008-09-25 修回日期:2009-06-16 出版日期:2009-08-01 发布日期:2010-12-29
  • 通讯作者: 黄和1 E-mail:biotech@njut.edu.cn
  • 基金资助:

    江苏省自然科学基金项目(BK2005114);国家自然科学基金项目(20576054);
    国家“863”计划项目(2006AA02Z240)

Mixture Design Optimization of Cellulase Complex Formulation

LIN Zeng-xiang1,ZHANG Hong-man2,YAN Li-shi1,CHEN Jing-wen1,HU Ling1,YU Wen-tao1,HUANG He1,*   

  1. 1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Pharmacy and Life Science, Nanjing University of
    Technology, Nanjing 210009, China;2. Department of Applied Chemistry, Nanjing University of Technology, Nanjing 210009, China
  • Received:2008-09-25 Revised:2009-06-16 Online:2009-08-01 Published:2010-12-29
  • Contact: HUANG He1,* E-mail:biotech@njut.edu.cn

摘要:

通过对三个不同来源的纤维素酶进行复配以提高纤维素酶的水解效率,以玉米秸秆酶水解液中还原糖浓度为评价指标,采用混料试验设计优选纤维素酶的复配条件并建立数学拟合方程。结果表明:最佳的酶复配条件为泽生纤维素酶与杰能科纤维素酶体积比1:1 时纤维素水解效率最高,比使用单一纤维素酶效率提高了39.5%。利用混料试验设计可以对纤维素酶复配条件进行优化。

关键词: 混料设计, 纤维素酶, 复配, 纤维素

Abstract:

Three different commercial cellulase preparations were used to prepare complex enzyme to increase the hydrolysis efficiency of cellulase. The formulation of cellulose complex was optimized by mixture design to attain the maximum concentration of reduced sugar in hydrolysate solution and a mathematical model describing the relationship of concentration of reduced sugar with volumes of mixed cellulases was developed. A mixture of Zesheng cellulase and Genencor cellulase at a volume ratio of 1:1 resulted in the maximum hydrolysis efficiency higher than that of individual cellulase by 39.5%. Mixture design is available for the optimization of cellulase complex formulation.

Key words: mixture design, cellulase, complex formulation, cellulose

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