食品科学 ›› 2009, Vol. 30 ›› Issue (16): 98-101.doi: 10.7506/spkx1002-6630-200916016

• 工艺技术 • 上一篇    下一篇

响应曲面优化超声波提取灵芝多糖工艺研究

董艳红1,李姝婧1,郑惠华2,李 雪1,陈贵堂3,赵立艳1 ,* ,辛志宏1,胡秋辉1   

  1. 1.南京农业大学食品科技学院 2.江苏通惠生物科技有限公司 3.中国药科大学药学院
  • 收稿日期:2009-03-11 修回日期:2009-06-16 出版日期:2009-08-15 发布日期:2010-12-29
  • 通讯作者: 赵立艳 E-mail:zhlychen@njau.edu.cn
  • 基金资助:

    现代农业产业技术体系建设专项资金资助项目;“十一五”国家科技支撑计划项目(2008BADA1B06);
    中农南农合作基金项目(Y200720)

Optimization of Ultrasonic-assisted Extraction Technology of Ganoderma lucidum Polysaccharides by Response Surface Methodology

DONG Yan-hong1,LI Shu-jing1,ZHENG Hui-hua2,LI Xue1,CHEN Gui-tang3,ZHAO Li-yan1,*, XIN Zhi-hong1, HU Qiu-hui1   

  1. 1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China;
    2. Jiangsu Tonghui Bio-technology Co. Ltd., Nantong 226100, China;
    3. School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China
  • Received:2009-03-11 Revised:2009-06-16 Online:2009-08-15 Published:2010-12-29
  • Contact: ZHAO Li-yan E-mail:zhlychen@njau.edu.cn

摘要:

采用响应曲面法对影响灵芝多糖提取得率的3 个主要影响因素即超声功率、超声时间和水料比进行优化。利用Design Expert 软件分析表明:灵芝多糖提取在超声功率680W、超声时间17min、水料比28:1 的最佳工艺条件下,得率达(20.79 ± 0.11)mg/g,实测结果与响应面拟和所得方程的预测值符合良好。

关键词: 灵芝多糖, 响应曲面, 超声波提取

Abstract:

Three key factors affecting the extraction yield of Ganoderma lucidum polysaccharides such as ultrasonic power, ultrasonic extraction time and ratio of water to solid were optimized by response surface methodology. Design Expert analysis indicated that the best ultrasonic power, ultrasonic extraction time and the ratio of water to solid were 680 W, 17 min and 28:1, respectively. Under these conditions, the yield of polysaccharide was (20.79 ± 0.11) mg/g, which was consistent with the predicted value.

Key words: Ganoderma lucidum polysaccharides, response surface methodology, ultrasonic extraction

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