食品科学 ›› 2010, Vol. 31 ›› Issue (15): 159-162.doi: 10.7506/spkx1002-6630-201015034

• 基础研究 • 上一篇    下一篇

7S 伴大豆球蛋白及其糖基化产物对大豆11S 球蛋白热聚集的影响

孙炜炜,于淑娟*   

  1. 华南理工大学轻工与食品学院
  • 收稿日期:2010-03-25 修回日期:2010-07-11 出版日期:2010-08-15 发布日期:2010-12-29
  • 通讯作者: 于淑娟* E-mail:lfshjyu@scut.edu.cn
  • 基金资助:

    国家自然科学基金项目(20776050);广东省自然科学基金项目(7006508)

Effects ofβ-Conglycinin and Glycatedβ-Conglycinin on the Thermal Aggregation of Glycinin

SUN Wei-wei,YU Shu-juan*   

  1. College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China
  • Received:2010-03-25 Revised:2010-07-11 Online:2010-08-15 Published:2010-12-29
  • Contact: YU Shu-juan* E-mail:lfshjyu@scut.edu.cn

摘要:

研究7S 伴大豆球蛋白(β - 伴大豆球蛋白)及其糖基化产物对大豆11S 球蛋白热聚集的影响。从浊度、ξ -电位、粒度、SDS-PAGE 测定得出在30mmol/L Tris-HCl 缓冲溶液中(含β - 巯基乙醇),7S 球蛋白及其糖基化产物能够抑制11S 球蛋白的热聚集,并且糖基化产物的抑制效果比未糖基化的7S 球蛋白明显;7S 球蛋白及其糖基化产物抑制11S 球蛋白热聚集的机理不同,7S 球蛋白糖基化产物对11S 球蛋白热聚集的抑制不是由于电荷的作用。

关键词: 7S 伴大豆球蛋白, 大豆11S 球蛋白, 糖基化, 热聚集

Abstract:

The effects of β-conglycinin and glycated β-conglycinin on the thermal aggregation of glycinin was studied. Evidence was presented to suggest that β-conglycinin and glycated β-conglycinin could both prevent thermal aggregation of glycinin in 30 mmol/L Tris-HCl buffer solution (β-Me exsisted). Further, addition of glycatedβ-conglycinin prevented thermal aggregation of glycinin more obviously than β-conglycinin. The results also indicated that the inhibitory mechanism of glycated β-conglycinin on glycinin aggregation was not as same as that of β-conglycinin due to charge-charge interactions.

Key words: β-conglycinin, glycinin, glycosylation, thermal aggregation

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