食品科学 ›› 2010, Vol. 31 ›› Issue (17): 323-326.doi: 10.7506/spkx1002-6630-201017072

• 生物工程 • 上一篇    下一篇

真姬菇液体菌种培养基筛选和摇瓶发酵条件的研究

王丽娟,刘林德,卜庆梅,王爱云,王淑芳   

  1. 鲁东大学生命科学学院
  • 收稿日期:2010-06-27 出版日期:2010-09-15 发布日期:2010-12-29
  • 通讯作者: 王丽娟 E-mail:wanglijuan53@126.com

Screening of Optimal Liquid Medium for Hypsizigus marmoreus and Optimization of Its Shake Flask Incubation Conditions

WANG Li-juan,LIU Lin-de,BU Qing-mei,WANG Ai-yun,WANG Shu-fang   

  1. School of Life Sciences, Ludong University, Yantai 264025, China
  • Received:2010-06-27 Online:2010-09-15 Published:2010-12-29
  • Contact: WANG Li-juan E-mail:wanglijuan53@126.com

摘要:

采用摇瓶培养法对真姬菇液体菌种培养基筛选和发酵条件进行研究。研究表明,真姬菇液体菌种最佳组合培养基为:葡萄糖3g/100mL、玉米淀粉1g/100mL、黄豆粉1.5g/100mL、(NH4)2SO4 0.3g/100mL、KH2PO4 0.2g/100mL、MgSO4·7H2O 0.1g/100mL 和VB1 10mg/L。真姬菇摇瓶发酵最佳条件为:起始pH 值为6.0,500mL 锥形瓶装液量140~150mL,摇床转速180r/min,温度26℃,二级摇瓶发酵时间72h,接种量10%~12%。

关键词: 真姬菇, 培养基筛选, 发酵条件

Abstract:

In this study, medium composition and process conditions for the liquid-state incubation of Hypsizigus marmoreus in a shake flask were explored. The optimal medium composition was found to consist of 3 g/100 mL glucose, 1 g/100 mL corn starch, 1.5 g/100 mL soybean cake, 0.3 g/100 mL (NH4)2SO4, 0.2 g/100 mL KH2PO4, 0.1 g/100 mL MgSO4·7H2O and 10 mg/L Vitamin B1. Initial pH of 6.0, medium volume contained in a 500 mL flask of 140-150 mL, shaking speed of 180 r/min, incubation temperature of 26 ℃, incubation time of 72 h and inoculum size of 10%-12% were found optimal.

Key words: Hypsizigus marmoreus, medium selection, incubation conditions

中图分类号: