关键词: 蜂蜜, β-葡萄糖苷酶, 酶活性, 来源

Abstract:

The determination conditions of β-glucosidase activity in honey were investigated by using p-Nitrophany β-DGluopyranoside (pNPG) as substrate. The optimal reaction pH and temperature of β-glucosidase were 5.0 and 60 ℃, respectively. The enzymatic reaction curve at 37 ℃ and a substrate concentration of 30 mmol/L presented the best  inearity. This enzyme may be added into honey during harvest and processing and its activity can be an indicator for the freshness of honey.

Key words: honey, β-glucosidase, enzyme activity, source