关键词: 薤白, 抗菌物质, 提取

Abstract: Previous studies have showed that Allium macrostemon Bunge is a broad-spectrum antimicrobial plant. In the present study, crude aqueous extracts isolated from Allium macrostemon Bunge under different levels of water amount as well as extraction time, temperature and number were tested comparatively for their anti-Escherichia coli and anti-Staphyloccocus aureus activities, and the plant showed better inhibitory effect against Staphyloccocus aureus than against anti-Escherichia coli. To obtain highly active anti-Staphyloccocus aureus substances from Allium macrostemon Bunge, the above four extraction conditions were optimized by response surface methodology based on inhibition circle diameter against Staphyloccocus aureus. The optimum levels of water amount as well as extraction time, temperature and number were determined to be 2-fold volume, 3 h (each time), 32 ℃ and 4, respectively, and under these conditions an extract with an inhibition circle diameter of 13.98 mm was obtained. Therefore, Allium macrostemon Bunge has the potential to be developed into a natural food preservative.

Key words: Allium macrostemon Bunge, antibacterial substance, extraction