食品科学 ›› 2011, Vol. 32 ›› Issue (5 ): 143-147.doi: 10.7506/spkx1002-6630-201105032

• 生物工程 • 上一篇    下一篇

桑黄菌丝体多糖的分离纯化及理化性质分析

谢丽源1,张 勇2,*,郭 勇1,吴 翔1,彭卫红1,甘炳成1   

  1. 1.四川省农业科学院土壤肥料研究所 2.电子科技大学生命科学与技术学院
  • 收稿日期:2010-06-19 修回日期:2011-02-14 出版日期:2011-03-15 发布日期:2011-03-03
  • 通讯作者: 谢丽源 E-mail:xieliyuan77@163.com
  • 基金资助:
    四川省青年基金项目(2008ZQ026-072);四川省科技支撑计划项目(2008FZ0157)

Isolation, Purification and Physico-chemical Characteristics Analysis of Mycelial Polysaccharides from Phellinus baumii

XIE Li-yuan1, ZHANG Yong2,*,GUO Yong1,WU Xiang1,PENG Wei-hong1,GAN Bing-cheng1   

  1. 1. Institute of Soil and Fertilizer, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China;
    2. School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China
  • Received:2010-06-19 Revised:2011-02-14 Online:2011-03-15 Published:2011-03-03
  • Contact: XIE Li-yuan1 E-mail:xieliyuan77@163.com

摘要: 研究桑黄粗多糖的分离和纯化工艺,并对多糖组分进行理化性质分析。结果表明:经DEAE-52纤维素离子交换层析和Sephadex G-100凝胶过滤层析得到两个组分P-47000和P-8700;经高效液相色谱分析证明,两组分均为纯品,且不含蛋白质、核酸,为非淀粉类多糖,分子质量分别为4.74×104D和8.71×103D,均由鼠李糖、阿拉伯糖、木糖、葡萄糖、半乳糖组成,P-47000中各单糖物质的量比为3.47:1.99:1:63.27:13.44,P-8700中各单糖物质的量比为10.46:1:1.03:182.75:30.94。

关键词: 桑黄, 多糖, 纯化, 理化性质

Abstract: The isolation, purification and physi-cochemical characterization of mycelial polysaccharides from Phellinus baumii were studied. Two polysaccharide fractions were separated from crude deproteinized polysaccharides from Phellinus baumii mycelia by DEAE-52 cellulose column chromatography, followed by purification on Sephadex G-100 gel filtration column. The two fractions were determined by HPLC to contain neither protein nor nuclear acid and to be pure non-starch polysaccharides, their molecular weights were 4.74×104 D and 8.71×103 D, respectively, and they were both composed of rhamnose, arabinose, xylose, galactose and glucose in a molar ratio of 3.47:1.99:1:63.27:13.44 and 10.46:1:1.03:182.75:30.94, respectively.

Key words: Phellinus baumii, polysaccharide, purification, physico-chemical characteristics

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