灵芝多糖树脂法脱色工艺优化

doi:10.7506/spkx1002-6630-201116002

食品科学 ›› 2011, Vol. 32 ›› Issue (16): 5-10.doi: 10.7506/spkx1002-6630-201116002

灵芝多糖树脂法脱色工艺优化

罗玺1,2,3，唐庆九1,*，张劲松1，周帅1，吴迪1，刘艳芳1，王琪1,2，乔彦茹1，杨焱1，赵明文2

1.国家食用菌工程技术研究中心，农业部应用真菌资源与利用重点开放实验室，上海市农业遗传重点开放实验室，上海市农业科学院食用菌研究所 2.南京农业大学生命科学学院微生物系 3. 河北省食用菌研究所

出版日期:2011-08-25 发布日期:2011-07-26
基金资助:
国家“863”计划项目(2007AA10Z341)

Optimization of Decolorization Technology of Ganoderma lucidum Polysaccharides by Macroporous Resin Adsorption Method

LUO Xi1,2,3，TANG Qing-jiu1,*，ZHANG Jin-song1，ZHOU Shuai1，WU Di1，LIU Yan-fang1，WANG Qi1,2， QIAO Yan-ru1，YANG Yan1，ZHAO Ming-wen2

1. National Engineering Research Center of Edible Fungi, Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China；2. Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China；3. Institute of Edible Fungi of Hebei Province, Shijiazhuang 050035, China

Online:2011-08-25 Published:2011-07-26

摘要/Abstract

摘要： 利用大孔树脂对灵芝多糖进行脱色。比较10种大孔树脂在脱色方面的性能，以灵芝多糖的脱色率和多糖保留率为考察指标，结果发现D303树脂的脱色效果最佳，通过单因素试验和正交试验对D303树脂的灵芝多糖脱色各种工艺参数进行优化，得到D303树脂静态脱色的最佳参数为在样品上样质量浓度15mg/mL、pH6、脱色温度50℃、脱色时间7h条件下，灵芝多糖溶液的脱色率可达91.89%，多糖保留率75.28%；D303树脂动态脱色的最佳参数为上样流速3BV/h、每毫升树脂上样量150mg，此条件下灵芝多糖溶液的脱色率可达92.01%，多糖保留率71.85%。研究表明D303树脂适合应用于灵芝多糖的脱色工艺。

关键词: 灵芝, 多糖, 脱色, 树脂

Abstract: The purpose of this study was to develop a macroporous resin adsorption method for the decolorization of Ganoderma lucidum polysaccharides. Ganoderma lucidum polysaccharides with a molecular weight between 10 kD and 500 kD were obtained by extracting Ganoderma lucidum fruiting bodies twice with boiling water, concentrating and ultrafiltrating with different MWCO (10 kD and 500 kD) membranes. Ten types of macroporous resin were compared for their effectiveness in decolorizing the polysaccharides. Based on decolorization rate and polysaccharide retention rate, type D303 macroporous resin had the best decolorization performance. Process parameter optimization for the decolorization of Ganoderma lucidum polysaccharides by static adsorption of type D303 macroporous resin were performed using one-factor-at-a-time combined with orthogonal array design method. The optimum conditions for static decoloration of resin D303 were as follows: sample concentration 15 mg/mL, pH 6, decoloration temperature 50 ℃, and decoloration time 7 h. Under the optimal conditions, a decoloration rate of 91.89% and a polysaccharide retention rate of 75.28% were obtained. The optimum conditions for dynamic decoloration of resin D303 were as follows: flow rate 3 BV/h (1 BV=10 mL), and sample loading amount 150 mg/mL resin, resulting in a decoloration rate of 92.01% and a polysaccharide retention rate of 71.85%. These findings collectively indicated that the resin D303 was more suitable for the decoloration of Ganoderma lucidum polysaccharides.

Key words: Ganoderma lucidum, polysaccharide, decoloration, resin

中图分类号:

罗玺，唐庆九，张劲松，周帅，吴迪，刘艳芳，王琪，乔彦茹，杨焱，赵明文. 灵芝多糖树脂法脱色工艺优化[J]. 食品科学, 2011, 32(16): 5-10.

LUO Xi，TANG Qing-jiu，ZHANG Jin-song，ZHOU Shuai，WU Di，LIU Yan-fang，WANG Qi，QIAO Yan-ru，YANG Yan，ZHAO Ming-wen. Optimization of Decolorization Technology of Ganoderma lucidum Polysaccharides by Macroporous Resin Adsorption Method[J]. FOOD SCIENCE, 2011, 32(16): 5-10.

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灵芝多糖树脂法脱色工艺优化

Optimization of Decolorization Technology of Ganoderma lucidum Polysaccharides by Macroporous Resin Adsorption Method

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