食品科学 ›› 2012, Vol. 33 ›› Issue (3): 72-76.doi: 10.7506/spkx1002-6630-201203016

• 基础研究 • 上一篇    下一篇

高压微射流处理对大豆分离蛋白构象及功能特性的影响

沈 兰,王昌盛,唐传核   

  1. 华南理工大学轻工与食品学院
  • 出版日期:2012-02-15 发布日期:2012-02-14
  • 基金资助:
    国家自然科学基金项目(30972049)

Effect of High Pressure Microfluidization on Functional and Conformational Properties of SPI

SHEN Lan,WANG Chang-sheng,TANG Chuan-he   

  1. College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China
  • Online:2012-02-15 Published:2012-02-14

摘要: 通过测定溶解度、乳化性、疏水性和运用差示扫描量热仪分析(DSC)和圆二色光谱(CD)分析探讨不同压力微射流处理(HPM)对大豆分离蛋白(SPI)构象和功能特性的影响。HPM诱导不溶性蛋白聚合物解聚,增加SPI的溶解度、乳化活性指数和疏水性。DSC图谱结果表明,处理后的SPI变性温度基本维持不变,但变性焓值呈现下降趋势。CD光谱分析表明,HPM对SPI的二级、三级结构没有明显的影响。

关键词: 大豆分离蛋白, 高压微射流, 功能特性, 构象

Abstract: The effect of high pressure microfluidization (HPM) on functional properties and conformational properties of soy protein isolated(SPI) was investigated by measuring solubility, emulsification, hydrophobic properties, DSC thermogram and UV-CD spectrum. HPM could cause insoluble aggregates to dissociate, thus improving the solubility, emulsifying activity index(EAI) and hydrophobic properties of SPI. DSC analysis indicated Td remained basically unchanged, but ΔH tended to decline. UV-CD spectrum analysis showed that both the tertiary conformation and secondary structure of SPI were nearly unaffected by HPM treatment.

Key words: soy protein isolated (SPI), high pressure microfluidization, functional properties, conformation

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