食品科学 ›› 2012, Vol. 33 ›› Issue (3): 252-255.doi: 10.7506/spkx1002-6630-201203053

• 营养卫生 • 上一篇    下一篇

鮟鱇鱼骨对去卵巢骨质疏松大鼠的影响

芦红艳,李妍妍,王 奇,王 霞,苏秀榕   

  1. 1.宁波大学海洋学院 2.中国药科大学药学院
  • 出版日期:2012-02-15 发布日期:2012-02-14
  • 基金资助:
    宁波市科技局农业与社发领域重大科技项目(2010C03017-1);宁波市重点学科资助项目(B01345104900)

Effects of Lophius litulon Bone in Ovariectomized Rats with Osteoporosis

LU Hong-yan,LI Yan-yan,WANG Qi,WANG Xia,SU Xiu-rong   

  1. 1. School of Marine Sciences, Ningbo University, Ningbo 315211, China; 2. School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China
  • Online:2012-02-15 Published:2012-02-14

摘要: 目的:通过研究鮟鱇鱼骨粉对去卵巢大鼠骨质疏松的作用,确定鮟鱇鱼加工下脚料的使用价值,方法:雌性SD大鼠60只,按体质量随机分为6组,即假手术组、模型对照组、阳性对照组(乳酸钙1000mg/(kg·d))及鱼骨高(6636mg/(kg·d))、中(2100mg/(kg·d))、低(665mg/(kg·d))3个剂量组。手术后12d,持续灌胃12周后,测定股骨长度、股骨质量、子宫形态以及血清中Ca、P和碱性磷酸酶(APL)、谷草转氨酶(AST)、谷丙转氨酶(ALT)的水平。结果:各剂量组大鼠与模型对照相比股骨质量升高,所有大鼠的股骨长度、血清Ca和血清P水平差异无显著性,模型对照组的血清碱性磷酸酶水平APL、AST、ALT与假手术组相比明显升高(P<0.05或P<0.01),而各剂量组的APL、AST、ALT水平与假手术组差异不显著。同时各剂量组可以促进子宫内膜腺体发育和内膜上皮无纤毛细胞上的微绒毛生长。结论:鮟鱇鱼骨可改善去卵巢大鼠的血清生化指标,并促进子宫内膜腺体发育和内膜上皮无纤毛细胞上的微绒毛生长。

关键词: 鮟鱇鱼骨, 去卵巢大鼠, 子宫形态, 生化指标

Abstract: Purpose: To find out the effect of Lophius litulon bone on osteoporosis in ovariectomized rats. Methods: Sixty SD female rats were divided at random into six groups: sham-operation group, model control group, positive control group [1000 mg/(kg·d) calcium lactate] and low-, medium- and high-dose treatment groups [at the doses of 665, 2100 mg/(kg·d) and 6636 mg/(kg·d), respectively]. At 12 days after ovariectomy, the rats were administrated for 12 consecutive weeks and then killed for the determination of femoral length and weight, uterus shape, and the serum levels of Ca, P, APL, AST and ALT. Results: The three treatment groups showed an increase in femoral weight compared with the model control group, but no significant difference in femoral length and the serum levels of P and Ca was observed. The serum levels of APL, AST and ALT in the model control group were significantly higher those of the sham-operation group, but no significant difference existed among the treatment groups and sham-operation group. In addition, each treatment group could promote endometrial gland development and microvillar growth in endometrial gland nonciliated epithelial cells. Conclusion: Lophius litulon bone can improve the serum biochemical indices and promote microvillar growth in endometrial gland nonciliated epithelial cells.

Key words: Lophius litulon bone, ovariectomized rat, uterus shape, biochemical indices

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