高效液相色谱-串联质谱法测定动物肌肉组织中氨基甲酸酯类杀虫剂及其代谢物残留

doi:10.7506/spkx1002-6630-201204044

食品科学 ›› 2012, Vol. 33 ›› Issue (4): 209-213.doi: 10.7506/spkx1002-6630-201204044

高效液相色谱-串联质谱法测定动物肌肉组织中氨基甲酸酯类杀虫剂及其代谢物残留

邓龙，郭新东，何强，杨群华，沈玉栋，王弘

1.广东省食品质量安全重点实验室，华南农业大学食品质量与安全研究所 2. 广州市质量监督检测研究院 3.广东省农药检定所

出版日期:2012-02-25 发布日期:2012-02-14

Simultaneous Determination of Carbamate Insecticides and Metabolite Residues in Animal Muscle by High Performance Liquid Chromatography-Tandem Mass Spectrometry

DENG Long，GUO Xin-dong，HE Qiang，YANG Qun-hua，SHEN Yu-dong，WANG Hong

1. Key Laboratory of Food Quality and Safety of Guangdong Province, Research Institute of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China；2. Guangzhou Quality Supervision and Testing Institute, Guangzhou 510110, China；3. Guangdong Institute for the Control of Agrochemicals, Guangzhou 510500, China

Online:2012-02-25 Published:2012-02-14

摘要/Abstract

摘要： 建立动物组织中氨基甲酸酯类杀虫剂及其代谢物(共16种)残留的高效液相色谱-串联质谱分析方法。样品经乙腈提取、浓缩、净化，液相色谱串联质谱测定，内标法定量。16种杀虫剂在1.0～100μg/L范围内线性关系良好(r＞0.9959)；方法定量限为0.5～2.5μg/kg；样品添加5.0、10.0、20μg/kg时，加标回收率为71.4%～105.5%；相对标准偏差为3.2%～13.7%。该方法具有简便快捷、灵敏度高的特点，适用于动物肌肉中氨基甲酸酯类杀虫剂及其代谢物残留量的检测。

关键词: 氨基甲酸酯, 液相色谱串联质谱, 动物肌肉组织

Abstract: A high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (HPLC-ESI/MS/MS) method for rapid determination of 16 residual analytes including carbamate insecticides and their metabolites in animal muscles was established. Samples were extracted with acetonitrile, and the extract was then condensed, cleaned up, analyzed by reversed phase HPLC-ESI/MS/MS, and quantified by internal standard method. The calibration curves for the analytes exhibited a good linear relationship in the range of 1.0－100 μg/L with correlation coefficient higher than 0.9959. The limits of quantification (LOQ) ranged from 0.5 to 2.5 μg/kg. The recovery rates of the analytes from animal muscle sample across the spike levels of 5.0, 10.0μg/kg and 20 μg/kg were in the range of 71.4%－105.5% with relative standard deviation (RSD) varying from 3.2% to 13.7%. The method was accurate, sensitive, simple and suitable for the simultaneous analysis of carbamate insecticides and their metabolite residues in animal muscle.

Key words: carbamate, high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (HPLC-ESI/MS/MS), animal muscle

中图分类号:

TS207.3

邓龙，郭新东，何强，杨群华，沈玉栋，王弘. 高效液相色谱-串联质谱法测定动物肌肉组织中氨基甲酸酯类杀虫剂及其代谢物残留[J]. 食品科学, 2012, 33(4): 209-213.

DENG Long，GUO Xin-dong，HE Qiang，YANG Qun-hua，SHEN Yu-dong，WANG Hong. Simultaneous Determination of Carbamate Insecticides and Metabolite Residues in Animal Muscle by High Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. FOOD SCIENCE, 2012, 33(4): 209-213.

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高效液相色谱-串联质谱法测定动物肌肉组织中氨基甲酸酯类杀虫剂及其代谢物残留

Simultaneous Determination of Carbamate Insecticides and Metabolite Residues in Animal Muscle by High Performance Liquid Chromatography-Tandem Mass Spectrometry

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