食品科学 ›› 2012, Vol. 33 ›› Issue (7): 20-25.doi: 10.7506/spkx1002-6630-201207005

• 基础研究 • 上一篇    下一篇

常见6种杂粮与2种主粮的抗氧化活性比较研究

徐元元,国旭丹,贺丽霞,王 敏*   

  1. 西北农林科技大学食品科学与工程学院
  • 出版日期:2012-04-15 发布日期:2012-04-20
  • 基金资助:
    “十二五”国家荞麦、燕麦产业技术体系建设基金项目(CARS-08); 校国际科技合作项目启动资金项目(A213021007)

Comparison of Antioxidant Activity between Six Kinds of Minor Cereals and Two Kinds of Staple Food Grains

XU Yuan-yuan,GUO Xu-dan,HE Li-xia,WANG Min*   

  1. (College of Food Science and Engineering, Northwest A & F University, Yangling 712100, China)
  • Online:2012-04-15 Published:2012-04-20

摘要: 目的:比较常见6种杂粮:荞麦(甜荞、苦荞)、小米、糜米(粳性、糯性)、玉米、薏米、燕麦与2种主粮:大米、小麦食用部分的总酚、黄酮含量及其存在形式和抗氧化活性差异,并分析总酚、黄酮含量与抗氧化活性之间的相关性。方法:采用Folin-Ciocalteu法测定总酚含量,NaNO2-Al(NO)3测定黄酮含量;DPPH自由基法、ABTS+ ·法、β-胡萝卜素-亚油酸乳化液法和还原力法评价样品的抗氧化能力。结果:总酚含量由高至低为:苦荞、甜荞、燕麦、薏米、玉米、小米、粳性糜米、小麦、糯性糜米、大米,并且除小米、糯性糜米外其他样品提取物的多酚主要以自由态存在,占总酚的64.18%~98.70%;黄酮含量最高的为薏米、燕麦次之,且均多以自由态存在;杂粮的抗氧化活性高于主粮,其中苦荞清除DPPH自由基能力、ABTS+ ·能力和总还原力最强,甜荞次之,而小米抑制β-胡萝卜素褪色能力最强;同时,抗氧化活性与总酚含量之间具有良好的线性关系,与黄酮含量的相关性不明显。结论:杂粮的总酚含量及总抗氧化活性高于主粮,其中苦荞最为突出,因此,推荐增加日常杂粮食用量以弥补主粮膳食中多酚物质含量过低的不足。

关键词: 杂粮, 抗氧化活性, 多酚, 黄酮

Abstract: Purpose: To compare the contents and existence form of polyphenols and flavonoids as well as antioxidant activities of edible parts between minor cereals (common buckwheat, tartary buckwheat, oat, coix, millet, corn and proso millet) and staple food grains (rice and wheat), and to explore the correlations between antioxidant properties and total phenolics or flavonoid contents. Methods: Total phenolic content was measured by Folin-Ciocalteu assay, and the content of total flavonoids was investigated by NaNO2-Al(NO)3 assay. Antioxidant properties were evaluated by DPPH free radical and ABTS+ · scavenging assay, reducing power and β-carotene-linoleic acid emulsion assay. Results: The content of total phenolics revealed a decreasing order as tartary buckwheat > common buckwheat> at>coix > corn>millet> proso millet> wheat > rice. Except for millet and proso millet, the major existence form of polyphenols was free phenolics (64.18%-98.70%). Additionally, the content of total flavonoids was the highest in coix, followed by oat, and existed mostly in a free form in both of them. The DPPH free radical and ABTS+ · scavenging capacity and reducing power of tartary buckwheat were higher than those of other cereals, while millet had the highest AAC value as determined by β-carotene-linoleic acid assay. In the present study, a significant correlation was observed between total phenolics and antioxidant activity. Conclusion: The antioxidant properties of minor cereals were higher than those of staple food grains, with the best antioxidant capacity in tartary buckwheat. Therefore, it can be suggested that minor cereals should be eaten more to improve the intake of dietary polyphenols, which are present in staple food grains in excessively small amounts.

Key words: minor cereal, antioxidant activity, phenolics, flavonoids

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