猪肉解冻过程中损伤型气单胞菌的检测及失活模型构建

doi:10.7506/spkx1002-6630-201207046

食品科学 ›› 2012, Vol. 33 ›› Issue (7): 219-222.doi: 10.7506/spkx1002-6630-201207046

猪肉解冻过程中损伤型气单胞菌的检测及失活模型构建

董庆利，梁娜，曾静，张浩博

上海理工大学医疗器械与食品学院

出版日期:2012-04-15 发布日期:2012-04-20
基金资助:
国家自然科学基金项目(308008640)

Detection and Inactivation Modeling of Injured Aeromonas spp. in Thawing Pork

DONG Qing-li，LIANG Na，ZENG Jing，ZHANG Hao-bo

(School of Medical of Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China)

Online:2012-04-15 Published:2012-04-20

摘要/Abstract

摘要： 研究猪肉在解冻过程中其表面的损伤型气单胞菌检测和生长模型的构建。以含质量分数0.6%酵母浸膏的胰酪胨大豆琼脂(TSAYE)为选择培养基，气单胞菌的损伤培养基中分别添加质量分数1.0%、1.5%、2.0%、2.5%、3.0%的NaCl，经差异显著性分析得出TSAYE+1.5% NaCl作为合适损伤培养基并用于后续研究。损伤型气单胞菌的数量采用普通培养基的菌落数与损伤培养基的菌落数之差来计数。猪肉在25℃解冻过程中，损伤型气单胞菌的修复时间大约为75min，其失活符合Polynomial模型(R2＝0.9937)及Boltzmann模型(R2＝0.9884)，通过模型的检验选择Polynomial模型拟合损伤型气单胞菌的失活过程。

关键词: 气单胞菌, 损伤, Boltzmann模型, Polynomial模型, 解冻猪肉

Abstract: In order to detect injured Aeromonas spp. and establish its inactivation model on the surface of thawing pork, TSAYE containing 0.6% yeast extract was selected and TSAYE supplemented with NaCl at various concentrations (1.0%, 1.5%, 2.0%, 2.5% and 3.0%) were used to culture Aeromonas spp. The results showed that TSAYE supplemented with 1.5% NaCl was the optimal injury medium. The number of injured Aeromonas spp. colonies was counted based on difference between ordinary medium and injury medium. This study indicated that the restoration time of Aeromonas spp. during the thawing process of pork at 25 ℃ was approximately 75 min, and the inactivation curve could be fitted with Polynomial model (R2 ＝0.9937) and Boltzmann model (R2 ＝0.9884), respectively. Moreover, polynomial model could better fit the inactivation process. This result can provide a theoretical reference for the detection and prediction of injured Aeromonas spp. in meat and meat products.

Key words: Aeromonas spp., injury, Boltzmann model, polynomial model, thawing pork

中图分类号:

TS201.3

董庆利，梁娜，曾静，张浩博. 猪肉解冻过程中损伤型气单胞菌的检测及失活模型构建[J]. 食品科学, 2012, 33(7): 219-222.

DONGQing-li，LIANGNa，ZENGJing，ZHANGHao-bo. Detection and Inactivation Modeling of Injured Aeromonas spp. in Thawing Pork[J]. FOOD SCIENCE, 2012, 33(7): 219-222.

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猪肉解冻过程中损伤型气单胞菌的检测及失活模型构建

Detection and Inactivation Modeling of Injured Aeromonas spp. in Thawing Pork

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