食品科学 ›› 2012, Vol. 33 ›› Issue (12): 8-11.doi: 10.7506/spkx1002-6630-201212002

• 工艺技术 • 上一篇    下一篇

正交试验优化超声辅助法提取三七有效成分工艺

周国燕,王春霞,桑迎迎,胡晓亮   

  1. 上海理工大学低温生物与食品冷冻研究所
  • 出版日期:2012-06-25 发布日期:2012-07-27
  • 基金资助:
    国家自然科学基金青年科学基金项目(50206013);上海市教委科研创新项目(09YZ230); 上海市重点学科建设项目(S30503);上海理工大学医疗器械与食品学院微创励志创新基金项目

Orthogonal Array Optimization of Ultrasonic-Assisted Extraction of Panax notoginseng Saponin Rg1

ZHOU Guo-yan,WANG Chun-xia,SANG Ying-ying,HU Xiao-liang   

  1. (Institute of Cryo-medicine and Food Refrigeration, Shanghai University of Science and Technogy, Shanghai 200093, China)
  • Online:2012-06-25 Published:2012-07-27

摘要: 研究超声辅助法提取三七有效成分的工艺。通过单因素试验研究乙醇溶液体积分数、料液比、超声波频率和提取时间对三七人参皂苷Rg1提取率的影响。在单因素试验基础上对4个因素安排L9(34)正交试验,得出影响三七有效成分人参皂苷Rg1提取率的先后次序为:乙醇体积分数>提取时间>料液比>超声频率。选出最佳提取工艺为:乙醇体积分数70%、料液比1:12(g/mL)、超声频率59kHz、提取时间60min。在此最佳工艺下,得到三七干燥后的人参皂苷Rg1含量为1.85%。

关键词: 三七, 超声辅助法, 人参皂苷Rg1, 提取

Abstract: The ultrasonic-assisted extraction of Panax notoginseng saponin Rg1 was optimized using an L9(34) orthogonal array design based on one-factor-at-a-time experiments. The effects of ethanol concentration, material-to-liquid ratio, ultrasonic frequency and extraction time on extraction efficiency were examined. Ethanol concentration had the highest effect on extraction efficiency, followed by extraction time and material-to-liquid ratio; the effect of ultrasonic frequency was the weakest. The optimal levels of ethanol concentraion, material-to-liquid ratio (g/mL), ultrasonic frequency and extraction time were 70%, 1:12, 59 kHz and 60 min, respectively. Under the optimized extraction conditions, the extraction yield of saponin from Radix Panax notoginseng was 1.85%.

Key words: Panax notoginseng, ultrasonic-assisted extraction, saponin Rg1

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