四川省猪肉源大肠杆菌和沙门氏菌的分离与耐药性分析

doi:10.7506/spkx1002-6630-201213043

食品科学 ›› 2012, Vol. 33 ›› Issue (13): 202-206.doi: 10.7506/spkx1002-6630-201213043

四川省猪肉源大肠杆菌和沙门氏菌的分离与耐药性分析

邹立扣，蒲妍君，杨莉，刘春华，肖平，罗燕，李蓓

四川农业大学都江堰校区微生物学实验室，森林资源保护与利用实验室

出版日期:2012-07-15 发布日期:2012-07-27
基金资助:
四川省教育厅重点资助项目(10ZA055)

Isolation and Drug Resistance Analysis of Escherichia coli and Salmonella spp. in Pork from Sichuan Province

ZOULi-kou，PUYan-jun，YANGLi，LIUChun-hua，XIAOPing，LUOYan，LIBei

(Laboratory of Microbiology, Laboratory of Forestry Resource Conservation and Utilization, Dujiangyan Campus of Sichuan Agricultural University, Dujiangyan 611830, China)

Online:2012-07-15 Published:2012-07-27

摘要/Abstract

摘要： 采集四川省鲜猪肉126份，选择性培养基分离大肠杆菌、沙门氏菌，划线纯化后，通过形态特性及16S rDNA序列对菌株进行鉴定，利用K-B法(CLSI)药敏试验检测大肠杆菌和沙门氏菌对17种抗生素的耐药性。结果表明：共获得大肠杆菌105株，沙门氏菌18株，检出率分别为83.33%和14.28%。大肠杆菌对各种抗生素的耐药率在0%～55.3%，对四环素、氨苄西林耐药率最高，分别为55.3%、52.3%，对其他抗菌药物的耐药率依次为卡那霉素(26.7%)＞链霉素(19.1%)＞环丙沙星(17.1%)＞诺氟沙星(14.3%)＞头孢噻吩(11.5%)＞氨苄西林/舒巴坦(4.8%)＞头孢曲松、头孢噻肟、氨曲南、庆大霉素(2.9%)＞头孢他啶(1.9%)>阿莫西林/克拉维酸(0.8%)＞丁胺卡那霉素(0.1%)＞亚胺培南、哌拉西林/他唑巴坦(0%)，共产生28种耐药谱，氨苄西林、四环素及氨苄西林/四环素谱型占优势。沙门氏菌对各种抗生素的耐药率在0%～55.5%，其中对四环素、氨苄西林、链霉素的耐药率最高，依次是55.5%、38.9%、27.8%，对其他抗菌药物的耐药率依次是：氨苄西林/舒巴坦、阿莫西林/克拉维酸(11.1%)＞头孢噻肟、卡那霉素、头孢噻吩、庆大霉素、环丙沙星、诺氟沙星(5.6%)＞头孢曲松、头孢他啶、亚胺培南、氨曲南、哌拉西林/他唑巴坦、丁胺卡那霉素(0%)，共产生13种耐药谱。四川省肉源大肠杆菌、沙门氏菌对各类抗生素已表现出一定的耐药性，虽耐药率相对较低，但多重耐药已经成为普遍现象，应引起足够重视，并加强对其监测。

关键词: 四川, 猪肉, 大肠杆菌, 沙门氏菌, 耐药性

Abstract: One hundred and twenty-six pork samples were collected in Sichuan province. Escherichia coli and Salmonella spp. were isolated using selective medium. The isolates were identified by morphological characteristics and confirmed by 16s rDNA sequence analysis. The susceptibility to seventeen antibiotics was performed according to the standard disk diffusion method recommended by the Clinical and Laboratory Standards Institute (CLSI). The results showed that 105 E. coli and 17 Salmonella spp. were obtained with detection frequency of 83.33% and 14.28%, respectively. Of 105 E. coli isolates, 55.3% and 52.3% were resistant to tetracycline and ampicillin, respectively. The resistance frequency to other antibiotics decreased in the following order: K (26.7%) > S (19.1%) > CIP (17.1%) > NOR (14.3%) > KF (11.5%) > SAM (4.8%) > CRO, CTX, ATM, CN (2.9%) > CAE (1.9%) > AMC (0.8%) > AK (0.1%) > IPM (0%), TZP (0%). There were 28 resistance profiles. Among these resistance profiles, AMP, TET and AMP/TET profiles were dominant. In addition, 55.5%, 38.9% and 27.8% of 17 Salmonella isolates were resistant to tetracycline, ampicillin and streptomycin, respectively. The resistance frequency to other antibiotics decreased in the following order: SAM, AMC (11.1%) > CTX, K, KF, CN, CIP, NOR (5.6%) > CRO, CAE, IPM, ATM, TZP and AK (0%). There were 13 resistance profiles. The isolates in pork from Sichuan province had appeared a certain drug-resistant frequency. Although the resistance frequency was lower than other reports, the multi-drug resistance was common in the isolates from pork, suggesting that the isolates from pork should be cautioned because they may constitute a reservoir of antibacterial resistance.

Key words: Sichuan province, pork, Escherichia coli, Salmonella spp., drug resistance

中图分类号:

邹立扣，蒲妍君，杨莉，刘春华，肖平，罗燕，李蓓. 四川省猪肉源大肠杆菌和沙门氏菌的分离与耐药性分析[J]. 食品科学, 2012, 33(13): 202-206.

ZOULi-kou，PUYan-jun，YANGLi，LIUChun-hua，XIAOPing，LUOYan，LIBei. Isolation and Drug Resistance Analysis of Escherichia coli and Salmonella spp. in Pork from Sichuan Province[J]. FOOD SCIENCE, 2012, 33(13): 202-206.

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四川省猪肉源大肠杆菌和沙门氏菌的分离与耐药性分析

Isolation and Drug Resistance Analysis of Escherichia coli and Salmonella spp. in Pork from Sichuan Province

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