食品科学

• 基础研究 • 上一篇    下一篇

菜籽蛋白水解物及其膜分离组分的降血压相关活性

何 荣1,鞠兴荣1,2,*   

  1. 1.江南大学食品学院,江苏 无锡 214122;2.南京财经大学食品科学与工程学院,江苏 南京 210046
  • 出版日期:2013-08-15 发布日期:2013-09-03
  • 通讯作者: 鞠兴荣
  • 基金资助:

    国家农业成果转化基金项目(2012GB24490611);江苏省自然科学基金项目(BK2001834)

Antihypertensive Properties of Enzymatic Hydrolysate of Rapeseed Protein and Its Ultrafiltered Fractions

HE Rong1,JU Xing-rong1,2,*   

  1. 1. School of Food Science and Technology, Jiangnan University, Wuxi 214122, China;
    2. College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210046, China
  • Online:2013-08-15 Published:2013-09-03
  • Contact: JU Xing-rong

摘要:

应用商业化蛋白酶Alcalase水解菜籽分离蛋白(RPI),并通过膜分离技术将水解物分离成4种不同分子质量大小的多肽组分(<1kD、1~3kD、3~5kD和5~10kD);评价水解物(RPH)和多肽组分的血管紧张素转化酶-Ⅰ(ACE)、肾素抑制活性和氧自由基吸收能力(ORAC)。结果表明:分子质量大小与菜籽肽的氧自由基吸附能力成负相关;<1kD的组分具有最大的ACE抑制活性(抑制率为(83.42±0.35)%)和氧自由基吸附能力;与膜分离组分相比,Alcalase水解物具有最高的肾素抑制活性。研究认为,菜籽蛋白Alcalase水解物及其<1kD的膜分离组分可能作为功能性成分用于降血压相关的功能食品和保健品的开发。

关键词: 菜籽蛋白, 膜分离, 血管紧张素转换酶-Ⅰ, 肾素, 氧自由基吸收能力

Abstract:

In this study, rapeseed protein isolate was hydrolyzed by alcalase and then separated into four different peptide
fractions (< 1 kD, 1 to 3 kD, 3 to 5 kD, and 5 to 10 kD) by membrane ultrafiltration. Renin and angiotensin converting
enzyme-Ⅰ (ACE) inhibitory activity, and oxygen radical absorbance capacity (ORAC) of the rapeseed protein hydrolysate
(RPH) and its ultra-filtered fractions were evaluated. The results showed a negative correlation between the molecular
weights of the fractions and their ORAC values. The fraction less than 1 kD revealed the highest ACE inhibitory activity
(83.42 ± 0.35)% and ORAC value, while the hydrolysate had the highest renin inhibitory activity when compared with
other fractions. Therefore, RPH and the fraction less than 1 kD can be used to develop functional and healthcare foods
which can lower blood pressure.

Key words: rapeseed protein isolate, membrane ultrafiltration, angiotensin converting enzyme-Ⅰ, renin, oxygen radical absorbance capacity

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