响应面法优化胡桃楸种仁壳总黄酮微波提取工艺

doi:10.7506/spkx1002-6630-201318007

食品科学

响应面法优化胡桃楸种仁壳总黄酮微波提取工艺

徐红艳，包怡红

1.东北林业大学林学院，黑龙江哈尔滨 150040；2.延边大学农学院，吉林延吉 133000

出版日期:2013-09-25 发布日期:2013-09-27

Optimization of Microwave-Assisted Extraction of Total Flavonoids from Seed Shell of Juglans mandshurica Using Response Surface Methodology

XU Hong-yan，BAO Yi-hong

1. School of Forestry, Northeast Forestry University, Harbin 150040, China；
2. Agricultural College, Yanbian University, Yanji 133000, China

Online:2013-09-25 Published:2013-09-27

摘要/Abstract

摘要：

以胡桃楸种仁壳为原料，研究微波提取其总黄酮的最佳提取工艺条件。以总黄酮得率和总还原力为指标，进行单因素试验，并在此基础上，选取乙醇体积分数、料液比、微波功率和微波时间为独立变量，采用响应面法进行优化并得到回归模型。结果表明：各变量影响主次顺序为料液比＞乙醇体积分数＞微波功率＞微波时间；经过优化后的提取工艺参数为：乙醇体积分数55%、料液比1:34(g/mL)、微波功率640W、微波时间13min，通过验证实验总黄酮得率为9.59mg/g，RSD为1.98%，该回归方程与实际情况拟合较好。提取液在7h内总黄酮含量和总还原力基本保持稳定。

关键词: 胡桃楸, 种仁壳, 总黄酮, 微波法, 响应面法

Abstract:

The objective of this study was to establish the optimal conditions for microwave-assisted extraction of total
flavonoids from seed shell of Juglans mandshurica. The extraction yield and total reducing power of total flavonoids
were investigated as a function of independent variables including ethanol concentration, solid-to-liquid ratio, microwave
output power and irradiation time by fitting a regression model. These variables were ranked in decreasing order of
importance as solid-to-liquid ratio, ethanol concentration, microwave output power and irradiation time. The optimized
extraction conditions were found to be 55% ethanol as the extraction solvent with a solid-to-liquid ratio of 1:34 (g/mL) and
microwave irradiation for 13 min with an output power of 640 W. The average extraction yield of total flavonoids obtained
from validation experiments was 9.59 mg/g with RSD of 1.98% (n = 3), and the experimental data could be fitted with
the developed regression model very well. Within 7 h, the total flavonoid content and total reducing power of the extract
obtained remained substantially.

Key words: Juglans mandshurica, seed shell, total flavonoids, microwave-assisted extraction, response surface methodology

徐红艳，包怡红. 响应面法优化胡桃楸种仁壳总黄酮微波提取工艺[J]. 食品科学, doi: 10.7506/spkx1002-6630-201318007.

XU Hong-yan，BAO Yi-hong. Optimization of Microwave-Assisted Extraction of Total Flavonoids from Seed Shell of Juglans mandshurica Using Response Surface Methodology[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201318007.

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响应面法优化胡桃楸种仁壳总黄酮微波提取工艺

Optimization of Microwave-Assisted Extraction of Total Flavonoids from Seed Shell of Juglans mandshurica Using Response Surface Methodology

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