食品科学
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熊海涛
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XIONG Hai-tao
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摘要:
在HAc-NaAc缓冲溶液中,镍(Ⅱ)对诺氟沙星的荧光强度有明显的淬灭作用,据此建立了一种测定镍(Ⅱ)含量的荧光光谱法。在优化实验条件下,其荧光淬灭强度与镍(Ⅱ)的浓度在5.00×10-7~1.00×10-5mol/L范围内呈良好的线性关系,检出限为2.1×10-7mol/L,相对标准偏差为1.5%(c=4.00×10-6mol/L,n=11)。该方法已成功应用于模拟废水与芹菜样中镍(Ⅱ)含量测定,回收率在99.60%~108.8%之间。
关键词: 镍(Ⅱ), 诺氟沙星, 荧光淬灭, 模拟废水样, 芹菜
Abstract:
In acetic acid/sodium acetate buffer solution, based on the obvious quenching effect of nickel (Ⅱ) on the fluorescent intensity of norfloxacin, a fluorescent spectroscopic method for the determination of trace nickel was developed. Under the optimal experimental conditions, the fluorescent quenching strength responded linearly with nickel (Ⅱ) concentration in 5.00 × 10-7 to 1.00 × 10-5 mol/L with a detection limit of 2.1 × 10-7 mol/L. The relative standard deviation was 1.5% (c = 4. 00 × 10-6 mol/L,n = 11). This method has been successfully applied to the determination of nickel (Ⅱ) in simulated waste water and celery samples, with recovery between 99.60% and 108.8%.
Key words: nickel (Ⅱ), norfloxacin, fluorescence quenching, simulated wastewater, celery
熊海涛. 荧光淬灭法测定模拟废水与芹菜中的微量镍[J]. 食品科学, doi: 10.7506/spkx1002-6630-201320046.
XIONG Hai-tao. Fluorescence Quenching Method for Determination of Trace Nickelin in Simulated Waste Water and Celery Samples[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201320046.
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链接本文: https://www.spkx.net.cn/CN/10.7506/spkx1002-6630-201320046
https://www.spkx.net.cn/CN/Y2013/V34/I20/217