低氧与低温胁迫对发芽玉米籽粒中γ-氨基丁酸富集的影响

doi:10.7506/spkx1002-6630-201501017

食品科学

低氧与低温胁迫对发芽玉米籽粒中γ-氨基丁酸富集的影响

尹永祺1,2，吴进贤1，刘春泉3，李大婧3，杨润强1，顾振新1,*

1.南京农业大学农业部农畜产品加工与质量控制重点开放实验室，江苏南京 210095；
2.扬州大学食品科学与工程学院，江苏扬州 225127；3.江苏省农业科学院农产品加工研究所，江苏南京 210014

出版日期:2015-01-15 发布日期:2015-01-16

Effects of Hypoxia and Cold Stress on γ-Aminobutyric Acid Accumulation in Germinating Maize

YIN Yongqi1,2, WU Jinxian1, LIU Chunquan3, LI Dajing3, YANG Runqiang1, GU Zhenxin1,*

1. Key Laboratory of Agricultural and Animal Products Processing and Quality Control, Ministry of Agriculture,
Nanjing Agricultural University, Nanjing 210095, China; 2. College of Food Science and Technology, Yangzhou University,
Yangzhou 225127, China; 3. Institute of Farm Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China

Online:2015-01-15 Published:2015-01-16

摘要/Abstract

摘要：

以玉米籽粒为实验材料，研究低氧胁迫下发芽时间、低温胁迫与回温解冻下的温度及时间对发芽玉米中γ-氨基丁酸（γ-aminobutyric acid，GABA）含量的影响，对其低氧和低温胁迫工艺进行了优化，同时对胁迫期间发芽玉米籽粒中GABA代谢酶活性的变化进行了研究。结果表明：玉米经低氧胁迫发芽72 h后，在－18 ℃冷冻6 h和25 ℃回温4 h条件下，发芽玉米中GABA含量增加29.9 倍，达到1.52 mg/g（以干质量计）；低氧胁迫下发芽玉米籽粒主要是通过GABA支路富集GABA的。玉米籽粒是富集GABA的良好原料，且低氧与低温胁迫是富集发芽玉米中GABA的有效方式。

关键词: 发芽玉米, 低氧胁迫, 低温胁迫, &gamma, -氨基丁酸, 富集

Abstract:

The effects of hypoxia and cold stress on γ-aminobutyric acid (GABA) accumulation of germinating maize were
investigated. The GABA content in germinating maize was monitored with respect to germination time under hypoxia stress
and freezing or chilling followed by exposure to room temperature (25 ℃). Results suggested that the optimal conditions for
GABA accumulation in 72 h-germinated maize during cold stress were freezing at -18 ℃ for 6 h followed by thawing for
4 h at 25 ℃. Under these conditions, the maximum GABA level (1.52 mg/g DW) was obtained, which was 30.9 folds higher
than that of the control. Moreover, it was also indicated that GABA synthesis in germinating maize was mainly achieved by
the GABA shunt. Maize can be used as a good raw material to accumulate GABA. Besides, hypoxia stress and cold stress
are confirmed as an effective way to accumulate GABA content in germinated maize.

Key words: germinating maize, hypoxia stress, cold stress, γ-aminobutyric acid, accumulation

中图分类号:

TS210.2

尹永祺1,2，吴进贤1，刘春泉3，李大婧3，杨润强1，顾振新1,*. 低氧与低温胁迫对发芽玉米籽粒中γ-氨基丁酸富集的影响[J]. 食品科学, doi: 10.7506/spkx1002-6630-201501017.

YIN Yongqi1,2, WU Jinxian1, LIU Chunquan3, LI Dajing3, YANG Runqiang1, GU Zhenxin1,*. Effects of Hypoxia and Cold Stress on γ-Aminobutyric Acid Accumulation in Germinating Maize[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201501017.

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低氧与低温胁迫对发芽玉米籽粒中γ-氨基丁酸富集的影响

Effects of Hypoxia and Cold Stress on γ-Aminobutyric Acid Accumulation in Germinating Maize

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