食品科学

• 工艺技术 • 上一篇    下一篇

薏米糠蛋白的提取工艺优化及其功能性质

林 莉1,秦礼康1,*,张 伟2   

  1. 1.贵州大学酿酒与食品工程学院,贵州 贵阳 550025;2.贵州鑫龙食品开发有限公司,贵州 安顺 561000
  • 出版日期:2015-01-25 发布日期:2015-01-16
  • 通讯作者: 秦礼康
  • 基金资助:

    贵州省重大专项(黔科合重大专项字[2013]6010-5;黔科合重大专项字[2014]6023);
    贵州省农业攻关项目(黔科合农G字[2012]4001);省市县三方合作项目(安西科合gzassxxlch[2012]3006(1);
    安市科合(2012)5)

Extraction and Functional Properties of Adlay Bran Protein

LIN Li1, QIN Likang1,*, ZHANG Wei2   

  1. 1. School of Liquor and Food Engineering, Guizhou University, Guiyang 550025, China;
    2. Food Development Co. Ltd. of Xinlong, Anshun 561000, China
  • Online:2015-01-25 Published:2015-01-16
  • Contact: QIN Likang

摘要:

以超临界脱脂薏米糠粉粕为研究对象,采用正交试验优化碱提蛋白工艺,并以酶法提取水相液蛋白为对照,比较两种蛋白的功能性质。结果表明,最佳的碱提条件为料液比1∶8、提取温度45 ℃、提取时间4 h、pH 9,平均提取率为63.75%;两种蛋白均含16 种氨基酸,且7 种必需氨基酸齐全;水酶法提取蛋白的溶解性、乳化性及其稳定性、起泡性均优于粉粕碱提蛋白。

关键词: 薏米糠蛋白, 提取, 功能性质

Abstract:

The alkali extraction of protein from defatted adlay bran was optimized using orthogonal array design and
the functional properties of the extracted protein were investigated in comparison to that obtained by aqueous enzymatic
extraction. The optimal extraction conditions were found to be extraction at 45 ℃ for 4 h with a solid-to-solvent ratio of 1:8
at pH 9, resulting in an average extraction yield of 63.75%. The products obtained by both extraction methods contained 16
amino acids including 7 essential ones. However, the protein obtained by aqueous enzymatic extraction was superior to that
obtained by this method regarding solubility, emulsifying properties and foaming properties.

Key words: adlay bran protein, extraction, functional properties

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