食品科学

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山西老陈醋对大鼠机体影响的代谢组学研究

李爱平1,2,范玛莉1,2,张 松3,秦雪梅1,杜冠华4,李震宇1,*   

  1. 1.山西大学 中医药现代研究中心,山西 太原 030006;2.山西大学化学化工学院,山西 太原 030006;3.山西省分析
    科学研究院核磁共振检测研究室,山西 太原 030006;4.中国医学科学院 北京协和医学院药物研究所,北京 100050
  • 出版日期:2015-12-15 发布日期:2015-12-24

Metabolomic Study of the Effect of Shanxi Aged Vinegar on Rats

LI Aiping1,2, FAN Mali1,2, ZHANG Song3, QIN Xuemei1, DU Guanhua4, LI Zhenyu1,*   

  1. 1. Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China;
    2. College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China; 3. Nuclear Magnetic Resonance
    Detecting Laboratory, Shanxi Academy of Analytical Sciences, Taiyuan 030006, China; 4. Institute of Materia Medica,
    Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China
  • Online:2015-12-15 Published:2015-12-24

摘要:

采用基于核磁共振氢谱(1H nuclear magnetic resonance,1H NMR)的代谢组学技术,结合代谢通路分析,对山西老陈醋对大鼠机体代谢组学的影响进行研究。结果表明:给予山西老陈醋后,大鼠的血清和尿液内源性代谢物发生明显变化,其中α-葡萄糖、β-葡萄糖、乳酸、柠檬酸、苹果酸、脂质和马尿酸等代谢物含量变化较大。代谢通路分析发现,山西老陈醋能显著影响大鼠机体的酮体合成与降解、淀粉和蔗糖代谢以及三羧酸循环和丁酸甲酯代谢,这些影响主要与能量代谢、糖代谢、脂肪代谢等过程密切相关。本研究从代谢组学的角度分析了山西老陈醋对大鼠正常机体代谢物的影响,为其抗疲劳、降糖、降脂等保健作用提供了研究依据。

关键词: 山西老陈醋, 代谢组学, 核磁共振氢谱, 多元统计分析, 代谢通路分析

Abstract:

Using 1H NMR-based metabolomic approach combined with metabolomics pathway analysis (MetPA), the
metabolic influence of Shanxi aged vinegar (SAV) on rats was carried out. The results indicated that metabolomic profiles of
SAV-treated rats differed greatly from those of normal controls, with a significant change observed in α-glucose, β-glucose,
lactate, citrate, malic acid, lipid, and hippurate. Four pathways including synthesis and degradation of ketone bodies and
metabolism of starch, sucrose, citrate cycle and methyl butyrate were regarded as the targeted metabolic pathways according
to MetPA. The results suggested that the metabolic alteration induced by Shanxi aged vinegar in rats was mainly involved
in energy metabolism, glucose metabolism and lipid pathways. This study revealed the effect of Shanxi aged vinegar on
metabolomic profiles of rats in a holistic way, which can lay the foundation for assessing anti-fatigue, hypoglycemic and
hypolipidemic functions of Shanxi aged vinegar.

Key words: Shanxi aged vinegar, metabolomics, 1H nuclear magnetic resonance, multivariate statistical analysis, metabolomics pathway analysis

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