食品科学

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茶多酚和乳清蛋白对冷藏鱼糜保鲜效果的影响

毕海丹,崔旭海*,王占一,于 滨   

  1. 枣庄学院生命科学学院,山东 枣庄 277160
  • 出版日期:2016-05-25 发布日期:2016-05-18

Effects of Tea Polyphenols and Whey Protein Isolate on Preservation of Surimi Quality during Cold Storage

BI Haidan, CUI Xuhai*, WANG Zhanyi, YU Bin   

  1. College of Life Science, Zaozhuang University, Zaozhuang 277160, China
  • Online:2016-05-25 Published:2016-05-18

摘要:

为探究茶多酚和乳清蛋白的添加对冷藏鱼糜保鲜效果的影响,以草鱼鱼糜为研究对象,设计4 组进行实验,A组为对照组,B组添加0.05%茶多酚,C组添加5%乳清蛋白,D组添加0.05%茶多酚+5%乳清蛋白。在4 ℃条件下分别冷藏0、1、3、5、7、9 d,通过测定总巯基含量、硫代巴比妥酸反应物值、过氧化值、挥发性盐基氮值、水分含量、凝胶强度的变化,研究茶多酚和乳清蛋白对冷藏鱼糜氧化及品质的影响。结果表明:与对照组相比,茶多酚的添加可以明显抑制蛋白氧化和脂肪氧化(P<0.05),抗氧化效果是D组>B组>C组;而乳清蛋白的添加可以明显减少鱼糜水分含量损失和凝胶劣化(P<0.05),其作用效果是D组>C组>B组。与冷藏最初相比,从3 d起,所有指标变化显著(P<0.05),且结果都显示同时添加茶多酚和乳清蛋白协同作用效果最显著;冷藏7 d时,D组的挥发性盐基氮值降低了4.3 mg/100 g,凝胶强度增加了260.07 g·mm,且保鲜效果显著(P<0.05)。因此,茶多酚和乳清蛋白共同应用于鱼糜制品生产中,将更利于提高保鲜效果。

关键词: 茶多酚, 乳清蛋白, 鱼糜, 冷藏, 保鲜效果

Abstract:

 This study aimed to examine the effects of tea polyphenols (TP) and whey protein isolate (WPI) on the
preservation of surimi quality during cold storage. In this experiment, grass carp surimi was stored at 4 ℃ and tested for
total sulfhydryl content, thiobarbituric acid-reactive substances (TBARS) value, peroxide value (POV), total volatile basic
nitrogen (TVB-N) value, water content, and gel strength at different storage times (0, 1, 3, 5, 7, and 9 d). The effects of tea
polyphenols and whey protein isolate on the oxidation and quality of surimi were investigated. Experiments were divided
into 4 groups. Group A was used as control, and groups B, C and D were added with 0.05% TP, 5% WPI, and 0.05%
TP + 5% WPI, respectively. The results showed that compared with the control group, the addition of tea polyphenols
could significantly (P < 0.05) inhibit protein and fat oxidation, and its antioxidant effect in three experimental groups was
in the order of D > B > C; the addition of whey protein isoalte could significantly (P < 0.05) reduce the loss of water and
gel degradation, this effect in three experimental groups was in the order of D > C > B. Compared with the beginning of
cold storage, there were significant changes (P < 0.05) in all measured parameters from 3 d onwards, and all the results
showed that the effect of the addition of tea polyphenols and whey protein isolate together was the most obvious, leading
to a decrease of 4.3 mg/100 g in TVB-N and an increase of 260.07 g·mm in gel strength after cold storage for 7 d, and the
preservative effect was significant (P < 0.05). Therefore, tea polyphenols and whey protein isolate could be used together in
the production of surimi products for improved preservative effect.

Key words: tea polyphenols, whey protein isolate, surimi, cold storage, preservative effect

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