小白菜硝酸盐含量与光照度及氮代谢关键酶的相关性

doi:10.7506/spkx1002-6630-201613033

摘要/Abstract

摘要：

采用土培实验研究不同品种小白菜（低富集硝酸盐小白菜品种“揭农四号春白菜”（JN4）、高富集硝酸盐小白菜品种“香港特选奶白菜”（HKTX））硝酸盐含量与光合系统参数及植物-土壤氮代谢关键酶的相关性。结果表明：随着光照度的增加，小白菜生物量总体呈增加趋势。与3 000 lx处理组相比较，光照度增加到6 000 lx和12 000 lx时，“HKTX”和“JN4”的地上部分干质量增加了10.51%～28.09%。随着光照度的增加，小白菜叶片硝酸盐含量显著降低。与3 000 lx处理组相比较，光照度增加到6 000 lx和12 000 lx时，“HKTX”和“JN4”的叶片硝酸盐含量降低了22.27%～43.32%，叶柄硝酸盐含量降低了8.21%～31.40%。两个供试品种小白菜叶片硝酸盐含量与净光合速率（Pn）成极显著负相关，相关系数分别为－0.686和－0.833（P＜0.01）；叶片硝酸还原酶活力与硝酸盐含量成显著负相关，相关系数分别为－0.719和－0.696（P＜0.05）；叶片、叶柄硝酸盐含量与土壤硝态氮含量成显著正相关，相关系数分别为0.692、0.726、0.676和0.796（P＜0.05）；土壤硝态氮含量与硝酸还原酶活性成极显著负相关，相关系数分别为－0.826和－0.832（P＜0.01）。

关键词: 硝酸盐含量, 光照度, 氮代谢关键酶, 相关性, 小白菜品种

Abstract:

Pot experiment was carried to explore the relationships of nitrate contents with photosynthetic system parameters
in two Chinese cabbage varieties (Brassica chinensis L.) (the low-nitrate variety ‘JN4’ and the high-nitrate variety ‘HKTX’)
under different light intensities (3 000, 6 000 and 12 000 lx) as well as with the key enzymes of nitrogen metabolism in
soil and plants. The results showed that the biomass of Chinese cabbage had an increasing trend with increasing light
intensity. The dry weight of shoots of ‘HKTX’ and ‘JN4’ increased by 10.51%?28.09% under light intensities of 6 000 and
12 000 lx as compared with that under 3 000 lx. The nitrate contents in leaves of the two Chinese cabbage varieties decreased
significantly with increasing light intensity. The nitrate contents in leaf blades and petioles of ‘HKTX’ and ‘JN4’ decreased
respectively by 22.27%?43.32% and 8.21%?31.40% under 6 000 and 12 000 lx as compared with those under 3 000 lx.
There were significantly negative correlations between nitrate content and either net photosynthetic rate (Pn) with correlation
coefficients of −0.686 and −0.833 (P < 0.01) or nitrate reductase (NR) activity in leave blades with correlation coefficients
of −0.719 and −0.696 (P < 0.05) for the varieties ‘HKTX’ and ‘JN4’, respectively. Significantly positive correlations existed
between the content of nitrate in either leaf blades or petioles and NO3^--N level in soil with correlation coefficients of 0.692,
0.726, 0.676 and 0.796 for ‘JN4’ and ‘HKTX’ (P < 0.05), respectively. Moreover, significantly negative correlations were
observed between soil NO3^--N content and nitrate reductase activity with correlation coefficients of −0.826 and −0.832
(P < 0.01), respectively.

Key words: nitrate content, light intensity, key enzymes of nitrogen metabolism, correlation, Chinese cabbage varieties

中图分类号:

陈永勤，冯勃，徐卫红，杨芸，迟荪琳，陈序根，王正银，谢德体. 小白菜硝酸盐含量与光照度及氮代谢关键酶的相关性[J]. 食品科学, doi: 10.7506/spkx1002-6630-201613033.

CHEN Yongqin, FENG Bo, XU Weihong, YANG Yun, CHI Sunlin, CHEN Xugen, WANG Zhengyin, XIE Deti. Relationship between Nitrate Contents of Chinese Cabbage (Brassica chinensis L.) and Light Intensity or Key Enzymes of Nitrogen Metabolism[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201613033.

参考文献

[1] ARCHER D L. Evidence that ingested nitrate and nitrite are beneficial

to health[J]. Journal of Food Protection, 2002, 65(5): 872-875.

[2] 都韶婷, 章永松, 林咸永, 等. 蔬菜积累的硝酸盐及其对人体健康

的影响[J]. 中国农业科学, 2007, 40(9): 2007-2014. DOI:10.3321/

j.issn:0578-1752.2007.09.021.

[3] MARCO T A, NEIL P B, GIOVANNA S C, et al. Combining social

learning with agro-ecological research practice for more effective

management of nitrate pollution[J]. Environmental Science and Policy,

2007, 10(6): 551-563. DOI:10.1016/j.envsci.2007.02.006.

[4] 高晗, 李斌, 宋静雅, 等. 新乡市蔬菜硝酸盐和亚硝酸盐污染状

况分析[J]. 河南科技学院学报(自然科学版), 2010, 38(1): 22-25.

DOI:10.3969/j.issn.1008-7516.2010.01.006.

[5] 黄敏, 余萃, 杨海舟, 等. 武汉市售典型蔬菜硝酸盐和亚硝酸盐污染

现状分析[J]. 安徽农业科学, 2010, 38(13): 6871-6873. DOI:10.3969/

j.issn.0517-6611.2010.13.099.

[6] 朱兰保, 盛蒂, 江升明. 蚌埠市售新鲜蔬菜的硝酸盐污染[ J ] .

光谱实验室, 2 0 1 3 , 3 0 ( 4 ) : 1 8 2 3 - 1 8 2 6 . D O I :1 0. 3 9 6 9 /

j.issn.1004-8138.2013.04.071.

[7] 李丽. 氮素浓度及形态对叶用萝卜硝酸盐含量的影响[D]. 武汉: 华

中农业大学, 2004: 5-35.

[8] 汪李平, 向长萍, 王运华. 白菜不同基因型硝酸盐含量差异的

研究[J]. 园艺学报, 2004, 31(l): 43-46. DOI:10.3321/j.issn:0513-

353X.2004.01.010.

[9] 沈明珠, 翟宝洁, 东惠茹, 等. 蔬菜硝酸盐累积的研究: Ⅰ.不同蔬菜

硝酸盐、亚硝酸盐含量评价[J]. 园艺学报, 1982, 9(4): 41-48.

[10] OLDAY F C, MAYNARD D N. A physiological basis for different

patterns of nitrate accumulation in two spinach cultivars[J]. Journal of the

American Society for Horticultural Science, 1976, 101(3): 217-219.

[11] 陈振德, 陈建美, 何金明, 等. 不同基因型大白菜硝酸盐积

累研究简报[J]. 山东农业科学, 2001(4): 9-11. DOI:10.3969/

j.issn.1001-4942.2002.02.009.

[12] 汪李平. 小白菜硝酸盐含量基因型差异及其遗传行为的研究[D]. 武

汉: 华中农业大学, 2001: 6-96.

[13] GRUDA N. Impact of environmental factors on product

quality of greenhouse vegetables for fresh consumption[J].

Critical Reviews in Plant Sciences, 2005, 24(3): 227-247.

DOI:10.1080/07352680591008628.

[14] 高祖民, 李式军, 索长江, 等. 不同光强下硝酸态氮与钼对叶菜类硝

酸盐积累的影响[J]. 园艺学报, 1987, 14(3): 192-196.

[15] 王忠全, 吴志鹏, 周子平. 氮源配比及光强对水培蔬菜生长和营养

品质的影响[J]. 上海蔬菜, 2005(1): 70-71.

[16] 周秋月, 吴沿友, 许文祥, 等. 光强对生菜硝酸盐累积的影响[J].

农机化研究, 2009(1): 189-192. DOI:10.3969/j.issn.1003-

188X.2009.01.057.

[17] 邹奇. 植物生理学实验[M]. 北京: 北京大学出版社, 1995: 27-29.

[18] OZAWA K, KAWAHIGASHI H. Positional cloning of the nitrite

reductase gene associated with good growth and regeneration ability

of calli and establishment of a new selection system for Agrobacterium

mediated transformation in rice (Oryza sativa L.)[J]. Plant Science,

2006, 170(2): 384-393. DOI:10.1016/j.plantsci.2005.09.015.

[19] 邹奇. 植物生理学实验指导[M]. 北京: 中国农业出版社, 2003:

125-126.

[20] 叶利庭, 吕华军, 宋文静, 等. 不同氮效率水稻生育后期氮代谢酶活

性的变化特征[J]. 土壤学报, 2011, 48(1): 132-140. DOI:10.11766/

trxb200906030242.

[21] 鲁如坤. 土壤农业化学分析方法[M] . 北京: 中国农业科技出版社,

1999: 156-157.

[22] 哈兹耶夫. 土壤酶活性[M]. 郑元洪, 周礼凯, 张德生, 等, 译. 北京:

科学出版社, 1980: 42-44; 100.

[23] 周晚来, 刘文科, 杨其长. 光对蔬菜硝酸盐累积的影响及其机理[J].

华北农学报, 2011, 26(增刊1): 125-130. DOI:10.7668/hbnxb.2011.

S2.029.

[24] 杨芸. 不同小白菜品种硝酸盐积累差异及及光照、温度和湿度调

控机理研究[D]. 重庆: 西南大学, 2015: 23-55.

[25] 朱为民. 蔬菜硝酸盐积累的基因型差异与营养调控及分子生物学

的研究[D]. 南京: 南京农业大学, 1997: 1-40.

[26] HUBER J L, HUBER S C, CAMPELLI W H. Reversible ligh/dark

modulation of spinach leaf nitrate reductase activity involved protein

phosphorylation[J]. Archives of Biochemistry & Biophysics, 1992,

296(1): 55-65. DOI:10.1016/0003-9861(92)90544-7.

[27] CHAMPIGNY M L. Integration of photosynthetic carbon and nitrogen

metabolism in higher plants[J]. Photosynthesis Research, 1995,

46(1/2): 117-127.

[28] 姚春霞, 陈振楼, 陆利民. 上海市郊菜地土壤和蔬菜硝酸盐

含量状况[J]. 水土保持学报, 2005(2): 85-88. DOI:10.3321/

j.issn:1009-2242.2005.01.021.

[29] 鲍俊丹. 土壤氮素硝化过程中亚硝态氮的累积及其影响因素研究[D].

杨凌: 西北农林科技大学, 2009: 23-51.