过氧乙酸消毒液处理动物性食品后氨基脲的生成量变化

doi:10.7506/spkx1002-6630-201617032

食品科学

过氧乙酸消毒液处理动物性食品后氨基脲的生成量变化

叶刚1，梁通雯1，淮文蓓2，谢冬冬1，沈金灿1,*，张毅1，康海宁1

1.深圳出入境检验检疫局食品检验检疫技术中心，深圳市食品安全检测技术研发重点实验室，广东深圳 518045；
2.广东药科大学中药学院，广东广州 528458

出版日期:2016-09-15 发布日期:2016-09-22

Differences of Semicarbazide Content in Animal-Derived Foods Disinfected by Peroxyacetic Acid Disinfectant

YE Gang1, LIANG Tongwen1, HUAI Wenbei2, XIE Dongdong1, SHEN Jincan1,*, ZHANG Yi1, KANG Haining1

1. Shenzhen Key Laboratory of Detection Technology R&D on Food Safety, Food Inspection and Quarantine Center of Shenzhen
Entry-Exit Inspection and Quarantine Bureau, Shenzhen 518045, China; 2. Traditional Chinese Medicine College,
Guangdong Pharmaceutical University, Guangzhou 528458, China

Online:2016-09-15 Published:2016-09-22

摘要/Abstract

摘要：

过氧乙酸作为一种高效、广谱的消毒剂在动物性食品的消毒中被广泛使用，但实验发现经过氧乙酸消毒液处理后的样品易产生氨基脲。为研究不同条件下经过氧乙酸消毒处理后食品中氨基脲生产量的差异，系统考察了过氧乙酸添加量、浸泡温度、浸泡时间、不同动物基质样品等因素对氨基脲生成量的影响。样品经过氧乙酸溶液处理后，加入过量的Na2S2O3除去未反应的过氧乙酸，在酸性条件下采用2-硝基苯甲醛进行衍生，乙酸乙酯提取，液相色谱-串联质谱仪测定，同位素内标法定量。结果表明：过氧乙酸添加量、浸泡温度及浸泡时间与氨基脲的生成量呈明显的正相关性。

关键词: 过氧乙酸, 消毒, 动物性食品, 氨基脲

Abstract:

Peroxyacetic acid is widely used in animal-derived foods disinfection since it is an effective and broad spectrum
disinfectant; however, our experiments have found that semicarbazide could be detected in samples treated by peroxyacetic
acid disinfectant. To study different contents of semicarbazide after treatment with peroxyacetic acid under different
conditions, the effects of peroxyacetic acid concentration, temperature, treatment time and different animal-derived foods
samples on the generation of semicarbazide were investigated. After samples were treated with peroxyacetic acid solution,
excessive Na2S2O3 was added to remove the unreacted peroxyacetic acid for the derivatization of semicarbazide into
2-nitrobenzaldehyde under acidic conditions. The preparation was extracted with ethyl acetate, and then the extract was
detected by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and quantified by isotope internal standard
method. The results showed that peroxyacetic acid concentration, temperature and treatment time had a significant positive
correlation with the yield of semicarbazide, respectively.

Key words: peroxyacetic acid, disinfection, animal-derived foods, semicarbazide

中图分类号:

TS207.5

叶刚，梁通雯，淮文蓓，谢冬冬，沈金灿，张毅，康海宁. 过氧乙酸消毒液处理动物性食品后氨基脲的生成量变化[J]. 食品科学, doi: 10.7506/spkx1002-6630-201617032.

YE Gang, LIANG Tongwen, HUAI Wenbei, XIE Dongdong, SHEN Jincan, ZHANG Yi, KANG Haining. Differences of Semicarbazide Content in Animal-Derived Foods Disinfected by Peroxyacetic Acid Disinfectant[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201617032.

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过氧乙酸消毒液处理动物性食品后氨基脲的生成量变化

Differences of Semicarbazide Content in Animal-Derived Foods Disinfected by Peroxyacetic Acid Disinfectant

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