食品科学 ›› 2019, Vol. 40 ›› Issue (10): 236-242.doi: 10.7506/spkx1002-6630-20180514-199

• 成分分析 • 上一篇    下一篇

鲟鱼传统发酵过程中挥发性风味物质的分析评价

赵 凤1,许 萍2,曾诗雨1,杨 兴1,*   

  1. 1.贵州省农业科学院水产研究所,贵州 贵阳 550025;2.通标标准技术服务(上海)有限公司农产食品部,上海 200030
  • 出版日期:2019-05-25 发布日期:2019-05-31
  • 基金资助:
    贵州省农业攻关项目(黔科合支撑[2018]2316);贵州省科研机构服务企业项目(黔科合平台人才[2016]5714);贵州省农科院青年基金项目([2018]05号);现代农业产业技术体系建设专项(CARS-46);贵州特色水产产业技术体系项目(GZCYTX2013)

Analysis of Volatile Compounds in Fermented Sturgeon

ZHAO Feng1, XU Ping2, ZENG Shiyu1, YANG Xing1,*   

  1. 1. Fisheries Research Institute, Guizhou Academy of Agricultural Sciences, Guiyang 550025, China; 2. Agriculture & Food Department, SGS-CSTC Standards Technical Services (Shanghai) Co. Ltd., Shanghai 200030, China
  • Online:2019-05-25 Published:2019-05-31

摘要: 以鲟鱼为原料,采用固相微萃取-气相色谱-质谱联用法,研究其发酵过程中挥发性风味物质种类和相对含量的变化情况,结合感觉阈值,采用相对气味活度值评价挥发性风味物质对总体风味的影响,探讨发酵过程中的关键性风味物质。分别检测新鲜鲟鱼,腌制鲟鱼,发酵5、10、20、25、35 d 7 个阶段的挥发性风味物质,结果表明:新鲜、腌制、发酵5、10、20、25、35 d 7 个阶段的挥发性风味物质种类分别为38、48、66、72、75、76 种和84 种。新鲜鲟鱼肉的主体风味物质为2,4-癸二烯醛、1-辛烯-3-醇、壬醛、癸醛、己醛等;腌制后的鲟鱼肉主体风味物质为壬醛、1-辛烯-3-醇、己醛、癸醛、柠檬烯等;发酵5 d鲟鱼肉的主体风味物质为1-辛烯-3-醇、柠檬烯、壬醛、乙酸乙酯、庚醇等;发酵10 d鲟鱼肉的主体风味物质为壬醛、1-辛烯-3-醇、乙酸乙酯、柠檬烯、癸醛等;发酵20 d鲟鱼肉的主体风味物质为壬醛、柠檬烯、1-辛烯-3-醇、癸醛、乙酸乙酯等;发酵25 d鲟鱼肉的主体风味物质为壬醛、柠檬烯、1-辛烯-3-醇、乙酸乙酯、癸醛等;发酵35 d鲟鱼肉的主体风味物质为乙酸乙酯、柠檬烯、壬醛、1-辛烯-3-醇、庚醇等。

关键词: 发酵鲟鱼, 气相色谱-质谱联用仪, 挥发性成分, 相对气味活度值

Abstract: The changes of volatile flavor compounds in fermented sturgeon sampled at different stages of the fermentation process were analyzed by solid phase micro-extraction (SPME) coupled to gas chromatography-mass spectrometry (GCMS). The key volatile flavor compounds were identified based on relative odor activity value (ROAV) as a function of odor threshold. A total of 38, 48, 66, 72, 75, 76 and 84 compounds were identified in fresh, salted, 5, 10, 20, 25 and 35 d fermented sturgeon respectively. 2,4-Decadienal, 1-octen-3-ol, nonanal, decanal, and hexanal were the key flavor compounds in the fresh sample. Nonanal, 1-octen-3-ol, hexanal, decanal, and D-limonene were the key flavor compounds in the salted sample. 1-Octen-3-ol, D-limonene, nonanal, hexanoic acid, ethyl ester, and 1-heptanol were the key flavor compounds in the 5 d fermented sample. Nonanal, 1-octen-3-ol, hexanoic acid, ethyl ester, D-limonene, and decanal were the key flavor compounds in the 10 d fermented sample. Nonanal, D-limonene, 1-octen-3-ol, decanal, hexanoic acid, and ethyl ester were the key flavor compounds in the 20 d fermented sample. Nonanal, D-limonene, 1-octen-3-ol, hexanoic acid, ethyl ester, and decanal were the key flavor compounds in the 25 d fermented sample. Hexanoic acid, ethyl ester, D-limonene, nonanal, 1-octen-3-ol, and 1-heptanol were the key flavor compounds in the 35 d fermented sample.

Key words: fermented sturgeon, gas chromatography-mass spectrometry (GC-MS), volatile flavor compounds, relative odor activity value

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