食品科学 ›› 2019, Vol. 40 ›› Issue (15): 277-284.doi: 10.7506/spkx1002-6630-20180822-234

• 专题论述 • 上一篇    下一篇

体外生物测定法在食品接触材料安全性评价中的应用研究进展

王文娟,蔡小芳,唐 洁,张喜荣,封 棣   

  1. 北京工商大学食品学院,北京市食品添加剂工程技术研究中心,食品质量与安全北京实验室,北京 100048
  • 出版日期:2019-08-15 发布日期:2019-08-26
  • 基金资助:
    国家自然科学基金面上项目(31871722);基础科研业务费“食品特色项目”(PXM2019_014213_000007)

Application of in Vitro Bioassays in Safety Evaluation of Food Contact Materials: A Review

WANG Wenjuan, CAI Xiaofang, TANG Jie, ZHANG Xirong, FENG Di   

  1. Beijing Engineering and Technology Research Center of Food Additives, Beijing Laboratory for Food Quality and Safety, School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048, China
  • Online:2019-08-15 Published:2019-08-26

摘要: 体外生物测定法是利用酵母、细菌、细胞等进行的生物体外短期毒性实验,可以有效针对生物体某一特异性效应,评价测试物的危害性以及探索其毒性作用机制。由于体外生物测定法可以提供食品接触材料迁移物(混合物)整体实际危害的综合信息,因此,近年来被越来越多地应用于食品接触材料的危害评估,尤其集中在细胞毒性、遗传毒性和内分泌干扰这3 类毒理学终点。本文重点综述了这3 类体外生物测定方法的基本原理,以及近20年来其在食品接触材料提取物危害评价中的应用研究进展,以期为今后食品接触材料生物学安全性评价的相关研究提供理论参考。

关键词: 食品接触材料, 安全性评价, 体外生物测定法, 细胞毒性, 遗传毒性, 内分泌干扰效应

Abstract: In vitro bioassays are a series of in vitro short-term toxicity tests utilizing yeasts, bacteria or cells, which allow the evaluation of the hazards of analytes and the exploration of the underlying toxicity mechanisms by effectively focusing on a specific effect in organisms. Owing to providing comprehensive information on the overall actual hazards of migrants (mixtures) from food contact materials, in vitro bioassays have been increasingly applied in the hazard assessment of food contact materials in recent years, especially focusing on three toxicological endpoints including cytotoxicity, genotoxicity and endocrine disruption effect. In this paper, the basic principles of the three kinds of bioassays and the progress made in their applications in the hazard assessment of food contact materials in the past two decades are reviewed. It is expected to provide a theoretical basis for future studies on the biological safety evaluation of food contact materials.

Key words: food contact materials, safety evaluation, in vitro bioassays, cytotoxicity, genotoxicity, endocrine disruption effect

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