食品科学 ›› 2019, Vol. 40 ›› Issue (22): 161-166.doi: 10.7506/spkx1002-6630-20181017-173

• 生物工程 • 上一篇    下一篇

Trichothecium roseum的酸碱属性及pH值对其胞外酶活性和致病性的影响

王振宇,胡慧敏,龚迪,张国军,Dov PRUSKY,毕阳   

  1. (1.甘肃农业大学食品科学与工程学院,甘肃 兰州 730070;2.甘肃农业大学园艺学院,甘肃 兰州 730070;3.以色列农业研究组织凡卡尼中心,中央区 Bet Dagan 50250)
  • 出版日期:2019-11-25 发布日期:2019-12-02
  • 基金资助:
    甘肃省自然科学基金项目(1610RJZA092);甘肃农业大学盛彤笙科技创新基金项目(GSAU-STS-1629)

Acid-Base Property of Trichothecium roseum and Effect of pH on Its Extracellular Enzyme Activities and Pathogenicity

WANG Zhenyu, HU Huimin, GONG Di, ZHANG Guojun, Dov PRUSKY, BI Yang   

  1. (1. College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China; 2. College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China; 3. The Volcani Center, Agricultural Research Organization, Bet Dagan 50250, Israel)
  • Online:2019-11-25 Published:2019-12-02

摘要: 目的:明确粉红单端孢(Trichothecium roseum)侵染果实的酸碱性质,测定3 种pH值孢子悬浮液接种对苹果果实病斑直径及胞外酶活性的影响。方法:用T. roseum接种“富士”苹果,测定果实病斑处的pH值变化。用pH值分别为3、5和7的孢子悬浮液接种果实,观察接种对果实病斑直径及病斑处果胶酶和纤维素酶活性的影响。结果:T. roseum接种后果实病斑处的pH值显著升高,由第0天的3.54升高至第12天的4.84,提高了36.7%。3 种pH值孢子悬浮液接种果实后,以pH 7接种的果实病斑直径最大,第9天时分别高出pH 5与pH 3接种的35.2%与68.0%。pH 7接种的果实病斑处具有最高的果胶酶及纤维素酶活性,第9天时的果胶甲酯酶、果胶甲基多聚半乳糖醛酸酶、多聚半乳糖醛酸酶活性分别为pH 3接种的2.95、1.61 倍和1.68 倍,为pH 5接种的2、1.25 倍和1.39 倍。此外,pH 7接种的果实病斑处纤维素酶和β-葡萄糖苷酶第9天的活性分别为pH 3接种的1.97 倍和1.64 倍,为pH 5接种的1.33 倍和1.3 倍。结论:T. roseum属碱化菌,中性或偏碱环境可提高该菌损伤接种苹果果实病斑处的胞外酶活性,增强其致病性。

关键词: 苹果果实, pH值, 致病性, 胞外酶

Abstract: Objective: The study aimed to define the acid-alkali property of Trichothecium roseum when inoculated to apple fruit and to determinate the effect of inoculation with its spore suspensions at three different pH values (3, 5 and 7) on lesion diameter and extracellular enzymes activities in apple fruit. Method: ‘Fuji’ apple fruit was artificially wounded and inoculated with T. roseum, and the pH change in lesion tissue was detected during incubation. Meanwhile, the lesion diameter and the activities of pectinase and cellulase in the inoculated fruit were determined. Results: The pH of lesion tissue increased by 36.7% from 3.54 on day 0 to 4.84 on day 12 after inoculation. The fruit inoculated with the spore suspension at pH 7 showed the largest lesion diameter, which was respectively 35.2% and 68.0% higher than that of the spore suspensions at pH 5 and pH 3 on day 9 after inoculation. The fruit inoculated with the spore suspension at pH 7 also had the highest activities of pectinase and cellulose in lesion tissue. The activities of pectin methylesterase, pectin methyl polygalacturonase and polygalacturonase were 2.95, 1.61 and 1.68 folds higher than those in the fruit inoculated with the spore suspension at pH 3, and were 2, 1.25 and 1.39 folds higher than those in the fruit inoculated with the spore suspension at pH 5, respectively. Moreover, the activities of cellulase and β-glucosidase in the fruit inoculated with the spore suspension at pH 7 were 1.97 and 1.64 folds higher than those in the fruit inoculated with the spore suspension at pH 3, and 1.33 and 1.3 folds higher than those in the fruit inoculated with the spore suspension at pH 5, respectively. Conclusion: T. roseum can produce alkaline substances. A neutral or alkaline environment increases the activities of extracellular enzymes in lesions, and enhances the pathogenicity of the pathogen.

Key words: Trichothecium roseum, apple fruit, pH, pathogenicity, extracellular enzymes

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