食品科学 ›› 2018, Vol. 39 ›› Issue (16): 201-207.doi: 10.7506/spkx1002-6630-201816029

• 生物工程 • 上一篇    下一篇

螺旋藻全蛋白与家族分类

王?娜1,2,向清豪1,2,赵肖荣1,2,李博生2,3,*,聂中良4   

  1. (1.北京林业大学生物科学与技术学院,北京 100083;2.北京林业大学 林业食品加工与安全北京市重点实验室,北京 100083;3.北京林业大学螺旋藻研究所,北京 100083;4.西南林业大学生命科学学院,云南?昆明 650224)
  • 出版日期:2018-08-25 发布日期:2018-08-17
  • 基金资助:
    公益性行业(林业)科研专项(201304605)

Family Classification of the Whole Proteins of Spirulina

WANG Na1,2, XIANG Qinghao1,2, ZHAO Xiaorong1,2, LI Bosheng2,3,*, NIE Zhongliang4   

  1. (1. College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China;2. Beijing Key Laboratory of Forestry Food Processing and Safety, Beijing Forestry University, Beijing 100083, China;3. Institute of Spirulina, Beijing Forestry University, Beijing 100083, China;4. College of Life Sciences, Southwest Forestry University, Kunming 650224, China)
  • Online:2018-08-25 Published:2018-08-17

摘要: 采用相对和绝对定量同位素标记实验的方法对螺旋藻全蛋白进行定性分析,并选用合理的统筹归类方法对其进行实用性归类。结果表明,共定性检测到螺旋藻蛋白质数为2?085?个,其中已知蛋白1?562?个;通过序列分类法将869?个蛋白共分成233?个蛋白超家族与87?个(均为已知蛋白)蛋白家族;根据蛋白的性质、结构和功能可分成7大类,发现蛋白超家族在抗氧化、抗癌、抗肿瘤、免疫调节等方面具有重要功能,可被广泛应用于食品、保健和医药等领域。由此可见,螺旋藻活性蛋白具有极大的开发前景。

关键词: 螺旋藻, 全蛋白, 家族分类, 相对和绝对定量同位素标记实验(iTRAQ), 功能分析

Abstract: This research adopted the isobaric tags for relative and absolute quantitation (iTRAQ) method for qualitative analysis of the whole proteins of Spirulina and employed a reasonable overall categorization method to categorize them according to practicability. The results demonstrated that a total of 2 085 Spirulina proteins were qualitatively identified including 1 562 known proteins. Totally, 869 annotated proteins could be classified into 233 protein superfamilies and 87 protein (all members are known proteins) families by sequence categorization. Based on their nature, structures and functions, these annotated proteins could be classified into 7 categories. It was found that the protein superfamilies had vital functions such as antioxidant, anticancer, antitumor and immunoregulation, and could be widely used in food, medicinal, chemical industry and other fields. Thus, active proteins of Spirulina have has promising prospects.

Key words: Spirulina, whole proteins, family classification, isobaric tags for relative and absolute quantitation (iTRAQ), functional analysis

中图分类号: