关键词: 反式脂肪酸, 反油酸, 转录组学, 信号通路, 生物信息学

Abstract: After being induced by elaidic acid, the differentially expressed genes in human umbilical vein endothelial cells were studied by transcriptional analysis and bioinformatics analysis at the transcriptional level. The results showed that a total of 95 genes were found to significantly changed, 31 of which were up-regulated while 64 were down-regulated. The bioinformatics analysis showed that a total of 772 genes were significantly differentially expressed during the biological evolution. There were 46 and 122 differentially expressed genes related to cell composition and molecular functions, respectively. A total of 93 signaling pathways were detected, of which 11 including terpenoid skeleton biosynthetic pathway, steroid biosynthetic pathway and fatty acid metabolic pathway were significantly changed. Based on the protein-protein interaction (PPI) and gene-gene interaction pathways, the target genes involved in different pathways including fatty acid synthase, STAT4 transcription factor and IL-23 cytokine were further analyzed.

Key words: trans fatty acid, elaidic acid, transcriptomics, signaling pathway, bioinformatics