食品科学 ›› 2018, Vol. 39 ›› Issue (22): 289-294.doi: 10.7506/spkx1002-6630-201822044

• 安全检测 • 上一篇    下一篇

加压毛细管电色谱法分离检测薯片中丙烯酰胺的含量

冯军1,2,肖晓茹3,李利军2,3,*,程昊2,3,黄文艺2,3,李彦青2,3,孔红星2,3   

  1. (1.广西科技大学医学院,广西?柳州 545005;2.广西科技大学 广西糖资源绿色加工重点实验室,广西高校糖资源加工重点实验室,广西?柳州 545006;3.广西科技大学生物与化学工程学院,广西?柳州 545006)
  • 出版日期:2018-11-25 发布日期:2018-11-21
  • 基金资助:
    广西自然科学基金项目(2014GXNSFAA118402);广西高等学校高水平创新团队及卓越学者计划资助项目

Separation and Determination of Acrylamide in Potato Chips by Pressurized Capillary Electrochromatography

FENG Jun1,2, XIAO Xiaoru3, LI Lijun2,3,*, CHENG Hao2,3, HUANG Wenyi2,3, LI Yanqing2,3, KONG Hongxing2,3   

  1. (1. College of Medicine, Guangxi University of Science and Technology, Liuzhou 545005, China; 2. Guangxi Key Laboratory of Green Processing of Sugar Resources, Key Laboratory for Processing of Sugar Resource of Guangxi High Education Institutes, Guangxi University of Science and Technology, Liuzhou 545006, China; 3. College of Biology and Chemical Engineering, Guangxi University of Science and Technology, Liuzhou 545006, China)
  • Online:2018-11-25 Published:2018-11-21

摘要: 建立加压毛细管电色谱法检测薯片中丙烯酰胺含量的方法。该方法采用EP-100-20/45-3-C18毛细管色谱柱(总长度45?cm,有效长度20?cm,直径100?μm,ODS填料3?μm)进行分离,并对流动相中有机相比例、磷酸盐缓冲溶液浓度、分离电压等实验条件进行考察和优化。结果表明:在流动相为乙腈-15?mmol/L?NaH2PO4(pH?4.7)缓冲盐溶液(15∶85,V/V),流动相流速为0.04?mL/min,分离电压为-2?kV,紫外检测波长为202?nm的条件下,薯片中的丙烯酰胺得到了良好地分离。方法在2.0~32?μg/mL范围内线性关系良好,相关系数为0.999?4。丙烯酰胺检出限(RSN=3)为0.30?μg/mL,平均回收率为95.3%~108.1%,相对标准偏差低于1.48%。该方法已经用于薯片中丙烯酰胺含量测定,结果良好。

关键词: 加压毛细管电色谱(pCEC), 薯片, 丙烯酰胺

Abstract: An efficient pressurized capillary electrochromatography (pCEC) method was developed for the determination of acrylamide in potato chips. The separation was performed on a reversed-phase EP-100-20/45-3-C18 capillary column (total length 45 cm, effective length 20 cm, diameter 100 μm, ODS packing 3 μm). Moreover, the mobile phase composition, the concentration of phosphate buffer, separation voltage and other experimental conditions were investigated and optimized, respectively. The mobile phase was composed of a mixture of acetonitrile and 15 mmol/L NaH2PO4 (pH 4.7) (15:85, V/V) at a flow rate of 0.04 mL/min. Under the optimal conditions (separation voltage ?2 kV, and UV detection wavelength 202 nm), the analyte was well separated. The linear range was 2.0–32 μg/mL with a correlation coefficient of 0.999 4. The detection limit (LOD) of acrylamide was 0.30 μg/mL (RSN = 3). The average recoveries were 95.3%–108.1%, with a relative standard deviation (RSD) less than 1.48%. The method has been used for the determination of acrylamide in potato chips with good results.

Key words: pressurized capillary electrochromatography (pCEC), potato chips, acrylamide

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