关键词: 炎症性肠病, 蓝莓提取物, 花色苷组成, 抗氧化能力, 炎症, 结肠癌

Abstract: Purpose: The objective of this study was to investigate the anthocyanin composition, antioxidant, anti-inflammatory and anticancer activities of blueberry extract (BE) and to unravel the possible underlying mechanism. Methods: The anthocyanin composition was analyzed by high performance liquid chromatography-electrospray ionization-tandem mass spectrometry. The antioxidant activity was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity. The levels of nitric oxide (NO), tumor necrosis factor-α (TNF-α), interlukin-6 (IL-6), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) were determined to explore the anti-inflammatory effect. The 3-(4,5)-dimethylthiahiazo(-z-yl)-3,5-di-phenytetrazoliumromide and Western blot analyses were used to determine the inhibitory effect on cell proliferation and apoptosis of BE at different concentrations. Results: Fourteen anthocyanins were identified in BE with strong antioxidant activity. BE remarkably reduced lipopolysaccharide (LPS)-induced production of inflammatory mediators such as NO, inflammatory cytokines such as IL-6 and TNF-α, and the expression of COX-2 and iNOS. BE could significantly inhibit the proliferation of HCT-116 cells in a dose- and time-dependent manner. Furthermore, BE treatment could result in a decrease in cyclin/cyclin-dependent kinase-2 (CDK2) and an increase in p53. Conclusion: BE has the potential to inhibit inflammation and colon cancer.

Key words: inflammation bowel diseases, blueberry extracts, anthocyanin composition, antioxidant activity, inflammation, colon cancer