食品科学 ›› 2023, Vol. 44 ›› Issue (9): 15-24.doi: 10.7506/spkx1002-6630-20220411-123

• 基础研究 • 上一篇    下一篇

利用青海弧菌Q67评估5 种农药的毒性作用

彭悦,文春露,杨春杰,蒋圣启,丁武   

  1. (西北农林科技大学食品科学与工程学院,陕西 杨凌 712100)
  • 出版日期:2023-05-15 发布日期:2023-05-24
  • 基金资助:
    陕西省重点研发计划项目(2021NY-153);陕西省技术创新引导专项(2021QFY10-06)

Toxicity Assessment of Five Pesticides Using Vibrio qinghaiensis sp. Q67

PENG Yue, WEN Chunlu, YANG Chunjie, JIANG Shengqi, DING Wu   

  1. (College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China)
  • Online:2023-05-15 Published:2023-05-24

摘要: 以青海弧菌Q67(Vibrio qinghaiensis sp. Q67)为指示生物,通过毒性单位和浓度加和模型评估抗蚜威(pirimicarb,PIR)、西维因(carbaryl,CAR)、草甘膦(glyphosate,GLY)、乐果(dimethoate,DIM)、乙酰甲胺磷(acephate,ACE)这5 种常见农药的毒性效应。结果表明,5 种农药的毒性大小依次为PIR>GLY>CAR>DIM>ACE。4 组二元混合体系联合毒性呈拮抗作用,其余组合为加和或协同效应。应用均匀设计射线法设计的7 条五元混合物射线中,4 条射线呈先协同后拮抗的作用趋势,3 条射线始终呈拮抗作用,且五元混合体系毒性与单一组分CAR的浓度比呈良好的正相关关系(r=0.947),综上,可建立线性模型对混合物毒性进行预测。本研究所建立的方法成本低、速度快、操作简单、便于携带,可作为一种潜在的农产品、食品农药残留快速检测的初筛方法。

关键词: 青海弧菌;联合毒性;农药;急性毒性;快速检测

Abstract: Vibrio qinghaiensis sp. Q67 was used as an indicator strain to study the toxic effects of five common pesticides, pirimicarb (PIR), carbaryl (CAR), glyphosate (GLY), dimethoate (DIM), and acephate (ACE). The toxicities were evaluated by toxic unit and concentration addition model. The results showed that the toxicity of the pesticides decreased in the following order: PIR > GLY > CAR > DIM > ACE. Four of the 10 binary mixtures of these five pesticides showed antagonistic toxic effects, while the remaining six showed additive or synergistic toxic effects. Among the seven five-component mixtures designed by uniform design ray, four showed synergistic and then antagonistic effects, while the remaining three showed a consistent antagonistic effect. The toxicity of the five-component mixtures was strongly positively correlated with the concentration of CAR (r = 0.947). Based on this result, a linear model was established to predict the toxicity of pesticide mixtures. This method has the advantages of low cost, rapidity, simplicity and convenience, which can be used as a potential preliminary screening method for rapid detection of pesticide residues in agricultural products and foods.

Key words: V. qinghaiensis sp. Q67; joint toxicity; pesticides; acute toxicity; rapid test

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