食品科学 ›› 2023, Vol. 44 ›› Issue (14): 54-62.doi: 10.7506/spkx1002-6630-20221105-052

• 食品化学 • 上一篇    下一篇

乳铁蛋白、表没食子儿茶素没食子酸酯、高甲酯果胶和β-环糊精四元复合物基高内相Pickering乳液的构建及表征

谢丽清,徐班萌,梁新红,李波,杨伟   

  1. (河南科技学院食品学院,河南?新乡 453003)
  • 出版日期:2023-07-25 发布日期:2023-08-11
  • 基金资助:
    河南省重点研发与推广专项(科技攻关)(212102110085;222102110040)

Fabrication and Characterization of High Internal Phase Pickering Emulsions Stabilized with Quaternary Complexes of Lactoferrin, Epigallocatechin-3-Gallate, High Methoxylated Pectin and β-Cyclodextrin

XIE Liqing, XU Banmeng, LIANG Xinhong, LI Bo, YANG Wei   

  1. (School of Food Science, Henan Institute of Science and Technology, Xinxiang 453003, China)
  • Online:2023-07-25 Published:2023-08-11

摘要: 利用乳铁蛋白(lactoferrin,LF)、表没食子儿茶素没食子酸酯(epigallocatechin-3-gallate,EGCG)、高甲酯果胶(high methoxylated pectin,HMP)和β-环糊精(β-cyclodextrin,β-CD)自组装构建四元复合物,表征复合物结构,并将其用于稳定高内相Pickering乳液(high internal phase Pickering emulsions,HIPPEs)。结果表明:与三元复合物相比,四元复合物具有较低的界面张力(降低约3 mN/m)和更加适宜的润湿性(接触角接近90°),表现出优异的稳定HIPPEs特性(乳滴粒径变小,均一性增强)。四元复合物的致密交联网络赋予HIPPEs更高的黏弹性(弹性最高提高至1.9 倍,黏性最高提高至5.3 倍)、质构特性、3D打印特性和冷藏稳定性。同时,β-CD能够通过干扰EGCG与LF或HMP的结合,显著提高HIPPEs的抗氧化特性(最高提高至2.1 倍)。另外,自组装顺序灵活调控着四元复合物的结构和乳化特性,其中LF-β-CD-EGCG-HMP四元复合物表现最为优异。本研究为蛋白质-多酚-双多糖四元复合物的构建及其在HIPPEs中的应用提供一定理论依据。

关键词: 乳铁蛋白;β-环糊精;四元复合物;高内相Pickering乳液;自组装顺序

Abstract: In this study, self-assembled quaternary complexes of lactoferrin (LF), epigallocatechin-3-gallate (EGCG), high methoxylated pectin (HMP) and β-cyclodextrin (β-CD) were constructed and characterized, and successfully utilized for stabilizing high internal phase Pickering emulsions (HIPPEs). In comparison with ternary complexes, the quaternary complexes had lower interfacial tension (reduced by about 3 mN/m) and more appropriate wettability (contact angle close to 90°), and were more effective in stabilizing HIPPEs (smaller droplet size and enhanced homogeneity). The dense cross-linking network of the quaternary complexes endowed HIPPEs with higher viscoelasticity (increasing elasticity and viscosity by up to 1.9 and 5.3 times, respectively), textural properties, 3D printing characteristics, and refrigeration stability. β-CD significantly improved the antioxidant activity of HIPPEs (up to 2.1 times) by interfering with the combination of EGCG with LF or HMP. In addition, the self-assembly sequence could flexibly regulate the structures and emulsifying properties of the quaternary complexes, with the LF-β-CD-EGCG-HMP complex having the most pronounced effect. In conclusion, this study provides a theoretical basis for the construction of protein-polyphenol-polysaccharide-polysaccharide complexes and their applications in HIPPEs.

Key words: lactoferrin; β-cyclodextrin; quaternary complexes; high internal phase Pickering emulsion; self-assembly sequence

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