食品科学 ›› 2001, Vol. 22 ›› Issue (1): 11-13.

• 基础研究 •    下一篇

枸杞多糖-2的抗羟基自由基氧化作用

 王建华, 张民, 甘璐, 张声华   

  1.  华中农业大学食品科技系; 山东农业大学理学院
  • 出版日期:2001-01-15 发布日期:2012-02-14

Study on Scavenging Effect of Lycium Barbarum Polysacch Aride-2 on the Oxidation Induced by Hydroxyl Radicals

 WANG  Jian-Hua, ZHANG  Min, GAN  Lu, ZHANG  Sheng-Hua   

  • Online:2001-01-15 Published:2012-02-14

摘要: 用水杨酸法检测羟基自由基(·OH)研究枸杞多糖组分2(LBP2)清除·OH的效果;用硫代巴比妥酸法测定小鼠肝丙二醛含量,用以DPH为荧光探剂的荧光偏振法测定小鼠肝线粒体膜流动性,以及用分光光度法测定小鼠红细胞溶血和肝线粒体膨胀程度研究LBP2的抗·OH氧化效果。结果表明,LBP2可以清除·OH,抑制·OH所致丙二醛的产生,抑制·OH所致膜流动性下降,减少·OH所致红细胞溶血,减轻·OH所致肝线粒体膨胀程度。可以得出LBP2具有抗·OH所致氧化的作用。

关键词: 枸杞, 多糖, 羟基自由基, 抗氧化

Abstract: The scavenging effect of LBP2 (one component of polysaccharides from Fructus Lycii,the fruit of Lycium barbarum L.) on hydroxyl radicals (·OH) was studied by salicylic acid method.To study the anti-oxidative effect of LBP2 on ·OH,the content of MDA in liver was measured by TBA assay ,the mitochondria membrance fluidity in liver was measured by the fluorescence polarization assay with DPH fluorescence probe,and the hemolysis extent of red blood cells and the swelling extent of mitochondria of liver were detected by spectrophotometric methods.The result showed that LPB2 had the ·OH scavenging activity.It could inhibit the generation of MDA,the decrease of mitochondria fluidity,the swelling of mitochondria,and the hemolysis of red blood cells induced by ·OH .The conclusion was that LBP2 had the effect of anti-·OH induced oxidation.

Key words: Lycium barbarum L. Polysaccharide Hydroxyl radical Anti-oxidation