食品科学 ›› 2003, Vol. 24 ›› Issue (5): 135-137.

• 分析检测 • 上一篇    下一篇

钼酸铵分光光度法测定葡萄籽提取物中的原花青素

 马亚军, 杨秉勤, 郎惠云   

  1. 西北大学化学系
  • 出版日期:2003-05-15 发布日期:2011-12-13

Determination of Functional Components in Grape Seeds by Ammonium Molybdate Spectrophotometry

 MA  Ya-Jun, YANG  Bing-Qin, LANG  Hui-Yun   

  • Online:2003-05-15 Published:2011-12-13

摘要: 本文提出了测定原花青素的新方法,它是基于原花青素与钼酸铵在弱酸性介质中反应生成黄色钼酸酯,反应产物在333nm波长处具有最大吸收。该方法的线性范围为5~110μg/ml,回收率为97.8%~102.5%,相关系数r=0.9995,检出限为2.7μg/ml.

关键词: 钼酸铵, 葡萄籽, 原花青素, 分光光度法

Abstract: A new method for deter mining oligomeric procyanidins by Ammonium Molybdate Spectrophotometry was studied.This basic reaction of oligomeric procyanidins with Ammonium molybdate to produce yellow ester molybdate would bepresented in a weak acid solution .The linear region was 5~110μg/ml, RSD=1.1%~1.3% and the recovery was 97.8%~102.5%. Thus the method was applied to determine oligomeric procyanidins to obtain a good result.

Key words: ammonium molybdate, grape seeds, oligomeric procyanidins, spectrometry