关键词: 微生物转谷氨酰胺酶, 球状蛋白质, 聚合, 催化机理

Abstract: The catalytic mechanism of microbial transglutaminase (MTGase) towards globular proteins was proposed for the firsttime.It was shown that there was an “induction phase”during the polymerization of globular proteins by MTGase,and thespatial structures of substrate proteins distinctly changed. The changes of structure improved the catalytic activity of MTGaseagainst globular proteins.β-lactoglubolin (β-LG) was chosen as the example. It was proved that catalysis of MTGase againtβ-LG did make the spatial structure ofβ-LG change remarkably.This catalytic mechanism of MTGase against globular proteinsproposed in this paper to a certein extent could explain the mechanism of modification of proteins by MTGase,and also providedirection for the more application of MTGase in different fields.

Key words: Microbial Transglutaminase (MTGase), globular proteins, polymerization, catalytic mechanism