食品科学 ›› 2005, Vol. 26 ›› Issue (1): 157-161.

• 工艺技术 • 上一篇    下一篇

火棘水溶性多糖的提取及分离纯化

张海生, 段玉峰, 周芳, 杨锋   

  1. 陕西师范大学食品工程系; 陕西师范大学食品工程系
  • 出版日期:2005-01-15 发布日期:2011-09-19

Extraction,Isolation and Purification of Water-soluble Polysaccharides from Pyracantha fortuneana

 ZHANG  Hai-Sheng, DUAN  Yu-Feng, ZHOU  Fang, YANG  Feng   

  1. Department of Food Engineering,Shaanxi Normal University
  • Online:2005-01-15 Published:2011-09-19

摘要: 本文对火棘水溶性多糖的提取工艺、分离纯化以及纯度鉴定作了研究。结果表明:火棘多糖的最佳提取工艺为90℃、9h、料液比为1:10,火棘多糖的提取率约为3.5%;通过分级沉淀、DE-52柱层析分离得到PP-A2、PP-A3、PP-A4和PP-B1、PP-B2、PP-B3六种组分;经SephadexG-200柱层析鉴定,PP-A2、PP-A3、PP-B2为均一多糖。

关键词: 火棘, 多糖, 提取, 分离纯化

Abstract: The extraction, isolation and purification of water-soluble Pyracantha fortuneana polysaccharides were studied. The results indicated that the optimal extraction technology of Pyracantha fortuneana polysaccharides were 90℃, 9h and 10:1(W/W) of water to its fruit. Extraction rate of the polysaccharides was 3.5%. Then through isolating it with fractional precipitation and DE-52 column chromatography, six glycoconjugates, PP-A2, PP-A3, PP-A4, PP-B1, PP-B2 and PP-B3, were obtained. Sephadex G-200 gel filtration chromatography showed that PP-A2, PP-A3, PP-B2 were pure.

Key words: Pyracantha fortuneana, polysaccharides, extraction, isolation and purification