食品科学 ›› 2005, Vol. 26 ›› Issue (10): 184-186.

• 分析检测 • 上一篇    下一篇

裙带菜孢子叶多糖含量的测定

 朱良, 王一飞, 朱艳梅   

  1. 暨南大学生物医药研究开发基地
  • 出版日期:2005-10-15 发布日期:2011-09-25

Determination of Polysaccharide in Sporophyll of Undaria pinnatifida Suringar

 ZHU  Liang, WANG  Yi-Fei, ZHU  Yan-Mei   

  1. Biomedical Research and Development Center of Jinan University
  • Online:2005-10-15 Published:2011-09-25

摘要: 用苯酚-硫酸比色法测定了裙带菜孢子叶中多糖的含量。测定波长489nm,多糖换算因子f=2.51;在5~80μg/ml范围内吸收度与被测物含量呈良好的线性关系,r=0.9998;多糖的平均回收率为98.57%,RSD为2.31%(n=5)。本法简便、准确、重现性好,可作为裙带菜孢子叶中多糖的含量测定方法。

关键词: 裙带菜孢子, 多糖, 苯酚-硫酸法, 含量测定

Abstract: The contents of polysaccharide in Sporophyll of Undaria pinnatifida Suringar. were assayed by phenol-H2SO4 colorimetry. The results were wavelength for measurement was 489nm and converting factor of polysaccharide 2.51; good linearity ranged from 5~80μg/ml,r=0.9998 and average recovery rate of polysaccharide 98.57% with 2.31% of RSD(n=5).The method used in this paper was simple, accurate and stable. It could be used for determination of the polysaccharide in leaves of sporophyll of Undaria pinnatifida Suringar.

Key words: sporophyll of Undaria pinnatifida Suringar, polysaccharide, anthrone-sulfuric acid determination