食品科学 ›› 2005, Vol. 26 ›› Issue (11): 207-209.

• 分析检测 • 上一篇    下一篇

HPLC法测定红辣椒油树脂中辣椒素的含量

 王鑫, 王兴国, 金青哲, 刘元法   

  1. 江南大学食品学院
  • 出版日期:2005-11-15 发布日期:2011-10-01

Determination of Capsaicinoids in Paprika Oleoresin by HPLC

 WANG  Xin, WANG  Xing-Guo, JIN  Qing-Zhe, LIU  Yuan-Fa   

  1. School of Food Science and Technology, Southern Yangtze University
  • Online:2005-11-15 Published:2011-10-01

摘要: 采用HPLC法对红辣椒油树脂中辣椒素的含量进行直接测定,方法简便,结果准确。辣椒素的平均回收率为99.87%,精密度0.51%。色谱条件为:色谱柱ZORBAXSB-C18(Φ4.6mm×150mm),流动相VMeOH:VH2O(65:35),流速1.0ml/min,柱温25℃,检测波长280nm。

关键词: 红辣椒油树脂, 辣椒素, HPLC

Abstract: HPLC was used to determinate the capsaicinoids content of paprika oleoresin directly. The metnod was simple and accurate. The average recoveries and RSD were 99.87% and 0.51% respectively. The conditions of HPLC were taken as following: column ZORBAX SB-C18(Φ4.6mm× 150mm), mobile phase VMeOH:VH2O(65:35), flow rate 1.0ml/min,column temperature 25℃ and wave length 280nm.

Key words: paprika oleoresin, capsaicinoids, HPLC