关键词: 食源性致病菌, 检测, 免疫学, PCR, 芯片

Abstract: Foodborne bacterial pathogens are defined to be bacterias that could lead to diseases through food consumption. The conventional detective methods on the base of culture have shown some disadvantages: complex operations, low specificities, variable sentitivitiesa ndt ime-consuming.R esently,t he useo fi mmunologicala nd molecularb iologicala ssaysh ave provided highly sensitive detection methods for specific pathogens in food samples. It conquers the disadvantages of conventional detective methods. The fomer include ELISA, immune-magnetism separate technique and immuno-gold- detection technique. The latter include DNA fingerprints which relied on PCR, multiplex-PCR, gene-chip techniques, quantitative PCR and real-time lightcycler PCR, etc.

Key words: foodborne bacterial pathogens, detection, immunology, PCR, chip