食品科学 ›› 2005, Vol. 26 ›› Issue (11): 65-68.

• 基础研究 • 上一篇    下一篇

固体培养蛹虫草核苷类次生代谢物的产率

 温鲁, 夏敏, 宋虎卫, 张乐, 周昊, 蒋洁, 袁丞墅   

  1. 淮阴师范学院生物系,淮阴师范学院分析测试中心
  • 出版日期:2005-11-15 发布日期:2011-10-01

Yield of Nucleotides Secondary Metabolite in Cordyceps militaris by Corn Culture

 WEN  Lu, XIA  Min, SONG  Hu-Wei, ZHANG  Le, ZHOU  Hao, JIANG  Jie, YUAN  Cheng-Shu   

  1. 1.Department of Biology, Huaiyin Normal College; 2.Center of Analysis and Testing, Huaiyin Normal College
  • Online:2005-11-15 Published:2011-10-01

摘要: 为提高固体培养蛹虫草核苷类代谢物的产率,用高效液相色谱法对培养物的虫草素和腺苷含量进行检测,从菌株、氮源、氮源水平、加水量、培养时间和光照等方面进行研究,在以20%豆粉为氮源、按1:1加营养液、接种Cm-2菌株、遮光培养35d的条件下,培养物的虫草素含量可达5.49mg/g,高于蛹虫草全草,为保健食品增加了新品种和新原料。

关键词: 大米, 蛹虫草, 虫草素, 腺苷, 固体培养, 高效液相色谱法, 保健食品

Abstract: In order to increase the yield of nucleotidte metabolite in Cordyceps militaris by corn culture, the strains, nitrogen resources and levels, water supply, culture period and illumination were assayed by determining the contents of cordycepin and adenosine in the culture produce by HPLC. The results showed that under 20% bean power as nitrogen resource, adding nutrient solution of equal volume, inoculating Cm-2 strain with light covering and culturing for 35 days, the content of cordycepin in the culture produce reached 5.49mg/g, significantly higher than that of the whole Cordyceps militaris. Therefore this study added newspecies and new material for health care food.

Key words: rice, Cordyceps militaris, cordycepin, adenosine, solid culture, HPLC, health care food